Assimilation of exogenous cyanide cross talk in Oryza sativa L. to the key nodes in nitrogen metabolism

Exogenous cyanide (CN^-) effects on nitrogen (N) uptake, transport, and assimilation in rice seedlings were investigated at the biochemical and molecular levels. Seedlings were treated with either a 2-d or 4-d supply of potassium cyanide (KCN) in the nutrient solution containing nitrate (NO₃^-) or ammonium (NH₄⁺). Although a KCN-induced increase was recorded in the activity of β-cyanoalanine synthase (β-CAS) in rice tissues of both NH₄⁺-fed and NO₃^--fed seedlings, the former showed a significantly greater assimilation rate for CN^- than the latter. The addition of KCN decreased NO₃^- uptake and assimilation, whereas a negligible impact was observed in NH₄⁺ treatments. Enzymatic assays showed a marked activities enhancement of glutamine synthetase (GS), glutamate synthase (GOGAT), and glutamate dehydrogenase (GDH) in NH₄⁺-fed seedlings after KCN exposure. Similarly, the rises occurring in nitrate reductase (NR) and GDH activity in NO₃^--fed seedlings were also detected after 2-d exposure to KCN, whereas a significant reduction of GS and GOGAT activities was determined. The results suggest that although exogenous KCN at moderate or high concentrations caused repressively effects on biomass growth of both NH₄⁺-fed and NO₃^--fed rice seedlings, the nontoxic concentration of KCN supplied can serve as a supplemental N source in plant nutrition and N metabolism.

Keywords: Cyanide; Nitrogen metabolism; Oryza sativa L.; β-cyanoalanine synthase.