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Genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus Tospovirus.

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Chrysanthemum stem necrosis virus (CSNV) is a member of a tentative tospovirus species. In this study, the complete genomic sequence of the Japanese CSNV isolate TcCh07A was determined. The L RNA is 8960 nt long and encodes the 331.0-kDa RNA-dependent RNA polymerase. The M RNA is 4828 nt long and

First Report of Natural Occurrence of Zucchini Lethal Chlorosis Tospovirus on Cucumber and Chrysanthemum Stem Necrosis Tospovirus on Tomato in Brazil.

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During a field survey in 1994, five cucumber (Cucumis sativus) cv. Hokushin plants showing symptom of yellowing, mottling, and vein banding on the leaves were collected from a commercial field of the Federal District. By electron microscopy, quasi-spherical particles with double membrane, typical

Antiviral Activity of Basidiomycetous Fungi Against Groundnut Bud Necrosis Virus in Tomato

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Tomato is an important vegetable crop which is severely affected by Groundnut bud necrosis virus (GBNV). Until now effective antiviral agents have not been reported for the management of necrosis disease caused by GBNV. Therefore, a study was undertaken to manage the necrosis disease caused by GBNV

Groundnut bud necrosis virus encoded NSm associates with membranes via its C-terminal domain.

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Groundnut Bud Necrosis Virus (GBNV) is a tripartite ambisense RNA plant virus that belongs to serogroup IV of Tospovirus genus. Non-Structural protein-m (NSm), which functions as movement protein in tospoviruses, is encoded by the M RNA. In this communication, we demonstrate that despite the absence

In silico analyses of molecular interactions between groundnut bud necrosis virus and its vector, Thrips palmi.

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Groundnut bud necrosis virus (GBNV) is an economically important tospovirus transmitted by Thrips palmi (Thysanoptera: Thripidae). The current understanding of thrips-tospovirus interactions is largely based on the tomato spotted wilt virus-Frankliniella occidentalis relationship. Only

Flagellin of Bacillus amyloliquefaciens works as a resistance inducer against groundnut bud necrosis virus in chilli (Capsicum annuum L.).

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Groundnut bud necrosis virus (GBNV), a member of the genus Tospovirus, has an extensive host range and is associated with necrosis disease of chilli (Capsicum annuum L.), which is a major threat to commercial production. Plant growth promoting rhizobacteria (PGPR) have been investigated for their

Effect of Temperature on Symptoms Expression and Viral RNA Accumulation in Groundnut Bud Necrosis Virus Infected Vigna unguiculata.

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Background

Groundnut bud necrosis virus (GBNV) (Tospovirus genus, Bunyaviridae family) infects the major crops of solanaceae, leguminosae and cucurbitaceae in India. Temperature is an important factor which influences the plant growth and development under

The complete nucleotide sequence and genome organization of the M RNA segment of peanut bud necrosis tospovirus and comparison with other tospoviruses.

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The M RNA of peanut bud necrosis virus (PBNV; synonym groundnut bud necrosis virus) is 4801 nucleotides in length. It comprised two ORFs in an ambisense organization and terminal inverted repeats. The 3' large ORF (3363 nucleotides in the virus-complementary strand) encoded a protein with a

Determination of MHC binding peptides and epitopes from non-structural movement (NSm) protein of groundnut bud necrosis virus.

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Groundnut bud necrosis virus (GBNV) is recognized as one of the most economically important viruses and is known to affect several crops including peanut, potato, tomato and soybean. For managing plant virus diseases, determination of their causal agents' identity at an early stage of crop is a

First Report of Groundnut bud necrosis virus in Iran.

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During the summer of 2001, mosaic, mottle, ring mosaic, stunting, and bud necrosis were observed in peanut fields (Arachis hypogaea cv. Gilan) in the Golestan Province of Iran. Mechanical inoculation of these samples caused necrotic local lesions on Vigna unguiculata cv. Mashad, necrosis on

Nucleotide sequence of the S and M RNA segments of a Groundnut bud necrosis virus isolate from Vigna radiata in India.

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Nucleotide sequence of the S and M RNA segments of a Groundnut bud necrosis virus isolate from Vigna radiata (mungbean isolate, GBNV-MB) was determined and compared with another isolate from Arachis hypogaea (groundnut isolate, GBNV-type). Comparative sequence analysis revealed that the genome

Complete genome sequence and phylogenetic relationships of tobacco streak virus causing groundnut stem necrosis disease in India.

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Tobacco streak virus (TSV, genus Ilarvirus family Bromoviridae) is known to cause stem necrosis disease (SND) in groundnut (Arachis hypogaea) since 2000 in Southern India. The TSV isolate infecting groundnut so far has not been characterized based on the complete genome

Isolation of an Isometric Virus Causing Sunflower Necrosis Disease in India.

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Sunflower necrosis disease (SND) is becoming a potential threat to sunflower (Helianthus annuus L.) cultivation in the Indian subcontinent. The disease was first recorded in parts of Karnataka state in 1997. Since then the disease has become increasingly important in Andhra Pradesh, Karnataka,

Investigations on the RNA binding and phosphorylation of groundnut bud necrosis virus nucleocapsid protein.

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Groundnut bud necrosis virus belongs to the genus Tospovirus, infects a wide range of crop plants and causes severe losses. To understand the role of the nucleocapsid protein in the viral life cycle, the protein was overexpressed in E. coli and purified by Ni-NTA chromatography. The purified N

First Report of Natural Infection of Luffa acutangula by Watermelon bud necrosis virus in India.

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In August 2002, ridge gourd (Luffa acutangula) plant samples exhibiting yellowing of leaves were collected from the experimental farm of Tamil Nadu Agricultural University, Coimbatore, India. Mechanical inoculations of ridge gourd seedlings using the above samples resulted in chlorotic spots on

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