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beta glucuronidase/horečka

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The ability of beta glucuronidase and a small dose of antigen to modify the anaphylactic reaction of previously sensitized mice has been further investigated. Protamine has an important effect on the immunological behavior of the enzyme. A trial on hay fever patients shows that the results in mice

[Beta-glucuronidase and acid phosphatase activity in children with rheumatic fever].

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Modulation of cellular glycosidase activity by hyperthermia.

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We examined the effect of 45 degrees C hyperthermia on the following glycosidases in CHO cells: beta-galactosidase, beta-hexosaminidase, beta-glucuronidase and alpha-mannosidase. Among these, lysosomal alpha-mannosidase exhibited the most dramatic response to hyperthermia with an increase in

Vectors for the genetic manipulation of African swine fever virus.

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Plasmid vectors designed to facilitate the genetic manipulation of African swine fever virus (ASFV) are described. Our results demonstrate that the beta-glucuronidase enzyme (GUS) can be used to follow gene expression in ASFV-infected cells. Infectious plaques formed by ASFV expressing GUS are

The structural protein p54 is essential for African swine fever virus viability.

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Protein p54, one of the most antigenic structural African swine fever virus (ASFV) proteins, has been localized by immuno-electron microscopy in the replication factories of infected cells, mainly associated with membranes and immature virus particles. Attempts to inactivate the p54 gene from ASFV
The authors examined 22 patients with rheumatic fever in whose cells (neutrophils, mononuclear cells, thrombocytes) and in the blood serum they noted the activity of lysosomal enzyme of beta-glucuronidase (BGU) and the concentration of protein in the cells of the peripheral blood. The activity of

Single and fractionated whole body hyperthermia in murine fibrosarcoma.

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The effects of single and multiple fractionated whole body hyperthermia (WBH) 41.5 degrees C on benzo(a) pyrene induced fibrosarcoma of mice were evaluated in terms of tumour response and systemic alterations of the host. While single exposure of WBH(S) 2 hrs, caused moderate inhibition of tumours

Monocyte function in familial Mediterranean fever.

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Monocytes derived from the peripheral blood of patients with familial Mediterranean fever (FMF) demonstrated a consistently lower phagocytic capacity (38-44%) for 125I-labelled Shigella flexneri when compared to monocytes from healthy subjects. Phagocytosis of both viable and killed Staphylococcus

A neutrophil lysozyme leak in patients with familial Mediterranean fever.

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Polymorphonuclear cells derived from the peripheral blood of patients with Familial Mediterranean Fever release more lysozyme in response to high temperature (42 degrees, 46 degrees C) than do control cells. No differences between the FMF and control cells were observed in the release of acid

The African swine fever virus thymidine kinase gene is required for efficient replication in swine macrophages and for virulence in swine.

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African swine fever virus (ASFV) replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including a thymidine kinase (TK) gene. Recombinant TK gene deletion viruses were produced by using two highly pathogenic isolates of ASFV through

Improvement of African swine fever virus neutralization assay using recombinant viruses expressing chromogenic marker genes.

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Antibody neutralization of African swine fever (ASF) virus measured by a plaque reduction assay presents frequent difficulties because of the absence or delay in plaque formation by many strains, especially low-passage viruses. To overcome this problem, a new ASF virus neutralization test has been

Sequential deletion of genes from the African swine fever virus genome using the cre/loxP recombination system.

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A method has been established to sequentially delete combinations of genes from the ASFV genome to test the effect on virus replication and host responses to infection. Initially the ASFV genes MGF505 2R and MGF505 3R and a truncated MGF360 9L gene were deleted from the genome of the tissue-culture

Thymoquinone Glucuronide Conjugated Magnetic Nanoparticle for Bimodal Imaging and Treatment of Cancer as a Novel Theranostic Platform.

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Theranostic oncology combines therapy and diagnosis and is a new field of medicine that specifically targets the disease by using targeted molecules to destroy the cancerous cells without damaging the surrounding healthy tissues.We aimed to develop a tool

Tumor-targeted delivery of 8-hydroxyquinoline.

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RIF-1 mouse tumors express high levels of beta-glucuronidase activity relative to most normal tissues. The high activity can be exploited for targeting specific drugs preferentially to tumor tissues. In this study we examined the kinetics of 8-hydroxyquinoline (8-OHQ) accumulation in tumor and in
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