lipoxygenase/kukuřice setá

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A novel plastidial lipoxygenase of maize (Zea mays) ZmLOX6 encodes for a fatty acid hydroperoxide lyase and is uniquely regulated by phytohormones and pathogen infection.

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Lipoxygenases (LOXs) are members of a large enzyme family that catalyze oxygenation of free polyunsaturated fatty acids into diverse hydroperoxide compounds, collectively called oxylipins. Although LOXs have been well studied in dicot species, reports of the genes encoding these enzymes are scarce

Biochemical Adaptations in Zea mays Roots to Short-Term Pb(2+) Exposure: ROS Generation and Metabolism.

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The present study investigated the effect of lead (0, 16, 40 and 80 mg L(-1) Pb2+) exposure for 3, 12 and 24 h on root biochemistry in hydroponically grown Zea mays (maize). Pb2+ exposure (80 mg L(-1)) enhanced malondialdehyde content (239%-427%), reactive carbonyl groups (425%-512%) and H2O2

Selective inhibition of jasmonic acid accumulation by a small α, β-unsaturated carbonyl and phenidone reveals different modes of octadecanoid signalling activation in response to insect elicitors and green leaf volatiles in Zea mays.

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BACKGROUND Plants often release a complex blend of volatile organic compounds (VOC) in response to insect herbivore damage. Among those blends of VOC green leaf volatiles (GLV) have been demonstrated to function as defence signals between plants, thereby providing protection against impending

[Features of lipoxygenase from cells of Mortierella species mushrooms].

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The specific activity of lipoxygenase from several strains of the zygomycete Mortierella varied from 1.02 to 2.02 microMol diene per min per mg protein). The enzyme equally used linoleic or arachidonic acid as a substrate. An increase in lipoxygenase activity was observed after adding corn oil to

Method to produce 9(S)-hydroperoxides of linoleic and linolenic acids by maize lipoxygenase.

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Seed from maize (corn) Zea mays provides a ready source of 9-lipoxygenase that oxidizes linoleic acid and linolenic acid into 9(S)-hydroperoxy-10(E),12(Z)-octadecadienoic acid and 9(S)-hydroperoxy-10(E),12(Z),15(Z)-octadecatrienoic acid, respectively. Corn seed has a very active

Transformations of alpha-linolenic acid in leaves of corn (Zea mays L.).

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Short incubation of [1-14C]alpha-linolenic acid with whole homogenate of leaves of corn (Zea mays L.) led to the formation of 4 major lipoxygenase products, i.e. 12-oxo-13-hydroxy-9(Z),15(Z)-octadecadienoic acid, 12-oxo-10,15(Z)-phytodienoic acid (12-oxo-PDA),

One-Step Bioconversion of Fatty Acids into C8-C9 Volatile Aroma Compounds by a Multifunctional Lipoxygenase Cloned from Pyropia haitanensis.

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The multifunctional lipoxygenase PhLOX cloned from Pyropia haitanensis was expressed in Escherichia coli with 24.4 mg·L-1 yield. PhLOX could catalyze the one-step bioconversion of C18-C22 fatty acids into C8-C9 volatile organic compounds (VOCs), displaying higher catalytic efficiency for eicosenoic

Dietary fish oil or lofrin, a 5-lipoxygenase inhibitor, decrease the growth-suppressing effects of coccidiosis in broiler chicks.

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Broiler chicks were fed a diet containing 4% of either corn oil or fish oil from 3 to 14 d of age. From Days 15 to 23, half of the chicks in each dietary treatment were fed Lofrin (an experimental 5-lipoxygenase inhibitor) at 33 micrograms/kg feed. The remaining chicks within each dietary treatment

Molecular characterization of L2 lipoxygenase from maize embryos.

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We investigated the expression and accumulation pattern of lipoxygenase isoforms throughout the maize plant life. Two forms of lipoxygenase L1 and L2 have been identified as acidic proteins of 100 kDa (pI 6.4) and 90 kDa (pI 5.5-5.7) which accumulate in dry embryos and in various organs of maize

Characterization of the maize lipoxygenase gene family in relation to aflatoxin accumulation resistance.

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Maize (Zea mays L.) is a globally important staple food crop prone to contamination by aflatoxin, a carcinogenic secondary metabolite produced by the fungus Aspergillus flavus. An efficient approach to reduce accumulation of aflatoxin is the development of germplasm resistant to colonization and

Diverted secondary metabolism and improved resistance to European corn borer (Ostrinia nubilalis) in maize (Zea mays L.) transformed with wheat oxalate oxidase.

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An alteration in the secondary metabolism of maize (Zea mays L.) genetically modified with the wheat oxalate oxidase (OxO) gene was observed using HPLC and fluorescence microscopy. Phenolic concentrations in the OxO lines were significantly increased, but DIMBOA synthesis was reduced due to a

Macrophage-mediated 15-lipoxygenase expression protects against atherosclerosis development.

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Oxidative modification of LDL increases its atherogenicity, and 15-lipoxygenase (15-LO) has been implicated in the process. To address this issue, we generated transgenic rabbits that expressed 15-LO in a macrophage-specific manner and studied their susceptibility to atherosclerosis development when

Up-regulation of chloroplastic antioxidant capacity is involved in alleviation of nickel toxicity of Zea mays L. by exogenous salicylic acid.

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A pot experiment was carried out to investigate the effect of exogenous salicylic acid (SA) on the growth, photosynthesis, oxidative stress and responses of chloroplastic antioxidant defense system of maize (Zea mays L.) plants grown in a nickel (Ni)-contaminated soil. The results indicate that

The maize lipoxygenase, ZmLOX10, mediates green leaf volatile, jasmonate and herbivore-induced plant volatile production for defense against insect attack.

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Fatty acid derivatives are of central importance for plant immunity against insect herbivores; however, major regulatory genes and the signals that modulate these defense metabolites are vastly understudied, especially in important agro-economic monocot species. Here we show that products and

Maize death acids, 9-lipoxygenase-derived cyclopente(a)nones, display activity as cytotoxic phytoalexins and transcriptional mediators.

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Plant damage promotes the interaction of lipoxygenases (LOXs) with fatty acids yielding 9-hydroperoxides, 13-hydroperoxides, and complex arrays of oxylipins. The action of 13-LOX on linolenic acid enables production of 12-oxo-phytodienoic acid (12-OPDA) and its downstream products, termed

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