The guava seed (Psidium guajava) nanoparticles as an antibacterial agent are prepared from guava seeds that have been washed, dried, and ground to powder of less than 1 mm diameter. The powder is reduced to nanoparticle size (less than 100 nm diameter) by adding the powder to a solution of concentrated hydrochloric acid (38% w/w) and stirring the mixture at 3000 rpm at room temperature. The resulting nanoparticles are filtered through a Millipore membrane filter and dried. Agar well diffusion studies showed significant antibacterial activity against various Gram positive and negative species commonly implicated in food contamination. Further testing showed the guava seed nanoparticles have significant antioxidant and radical scavenging content, suggesting that guava seed nanoparticles may serve as an antibacterial agent.

KING SAUD UNIVERSITY

King Saud University

Guava seed (Psidium guajava) nanoparticles as antibacterial agent

The present invention relates to a novel peptide extracted from guava (Psidium guajava) seeds, that provides bactericide activity, Preferentially against Gram-negative bacteria which are known to cause urinary, hospital, and intestinal tract infections (Proteus sp. And Klebsiella sp.). The peptide, that has the amino acid sequence RESPSSRMEC YEQAERYGYG GYGGGRYGGG YGSGRGQPVG QGVERSHDDN RNQPR, belongs to the class of glycine rich proteins and has approximately 5 kDa of molecular weight. The invention also relates to antibiotic compositions for human, veterinary and plant treatments. Alternatively, the peptide, or a functionally similar derivative, subjects of the present invention, can be used for transforming organisms aiming pathogen resistance, other adaptive advantages, as well as various properties, specially for plants and animals.

psidium guajava/baktericid

Guava seed (Psidium guajava) nanoparticles as antibacterial agent

Plant derived antibiotic peptide rich in glycine

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