[Analysis of chemical components of volatile oil from Paris polyphylla and their antibacterial activities].

OBJECTIVE

To analyze the chemical components of volatile oil from Paris polyphylla by GC-MS, and to investigate their antimicrobial activities.

METHODS

The chemical compositions of essential oil, which from Paris polyphylla obtained by supercritical carbon dioxide, were analyzed by GC-MS, and their antibacterial properties for five common pathogens were screened by filter paper method.

RESULTS

The components in the volatile oils were analyzed by GC-MS, and 75 compounds comprising for 98.46% of the essential oil were identified. The major components of essential oil were phthalic acid-isobutyl-hex-3-enyl ester, 9,12-octadecadienoic acid, 2 (1H)-naphthalenone, octahydro-4alpha-methyl-7-(1-methylethyl)-, glycerin, 9-octadecenoic acid-methyl ester, 1,2-benzenedicarboxylic acid-butyl-2-methylpropyl ester, bis(2-ethylhexyl) phthalate, 2-myristynoyl-glycinamide, ethanol, 2-(9,12) -octadecadienyloxy, hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester, dodecanoic acid, 10-methyl, methyl ester, diglycerol and 3,3-diethoxy-1-propanol; The bacteriostatic experiment results indicated that this volatile oil had strong inhibitory effects on Micrococcus, Aerobacter and Brevibacterium, whereas it had poor effects Escherichia coli and Proteus vulgaris.

CONCLUSIONS

The experiment provides a scientific basis for further development and utilization of Paris polyphylla.