[Analysis of serum metabonome of patients with breast cancer by gas chromatography-mass spectrometry].
Nøgleord
Abstrakt
OBJECTIVE
To analyze serum metabonome of patients with breast carcinoma by gas chromatography-mass spectrometry (GC-MS).
METHODS
Serum samples from 30 patients with breast carcinoma and 25 normal people were collected. Endogenous metabolites amino acids, fatty acids, saccharides and organic acids were analyzed after derivatization.
RESULTS
The injection precision and intra-day precision (RSD) were less than 14.4 %. The method displayed good recovery (80.7 %-118.0 % with RSD<12.0 %) and linearity (r(2)>0.9905). Samples were stable in 24 h at room temperature. The samples were stable through three freeze-thaw cycles (RSD<14.6 %). Alaine,oxalate and glutamine were found with frequency of 96.7 %, 100 % and 93.3 %, respectively, which indicated that they may be the potential biomarkers.
CONCLUSIONS
The method is stable and reliable, which may be helpful for the diagnosis of breast cancer as an auxiliary method.
