Draft genome analysis of the endophyte, Bacillus toyonensis COPE52, a blueberry (Vaccinium spp. var. Biloxi) growth-promoting bacterium.

In this work, we report an analysis of the draft genome of the blueberry (Vaccinium spp. var. Biloxi) growth-promoting endophyte Bacillus toyonensis, strain COPE52. The genome of COPE52 consists of a single 5,806,513 bp replicon, with a 35.1% G + C content. Strain COPE52 was strongly affiliated to B. toyonensis species, based on species delimitation cut-off values established for average nucleotide identity (> 95-96%), genome-to genome distance calculator (> 70%) and phylogenomic analysis. The RAST genomic annotation of the COPE52 strain revealed a total of 5979 total genes, including 5631 protein-coding genes, 11 rRNA genes, 5 ncRNAs, 81 tRNA genes, and 251 pseudogenes. To further validate the in silico analysis results, experiments were carried out to detect the production of indoleacetic acid, protease activity, and the emission of volatiles like acetoin, 2,3-butanediol and dimethyl disulphide as potential plant growth-promoting mechanisms. COPE52 also showed antifungal action against the grey mould phytopathogen, Botrytis cinerea, during in vitro bioassays. In addition, inoculation with strain COPE52 promoted growth biomass and chlorophyll content in blueberry plants (Vaccinium spp. var. Biloxi) under greenhouse conditions. To our knowledge, this is the first study showing genomic and experimental evidence of B. toyonensis as plant growth-promoting bacteria (PGPB).