Effect of dietary trans-stilbene oxide on hepatic and renal drug-metabolizing enzyme activities and bromobenzene-induced toxicity in male Sprague-Dawley rats.

The effect of dietary trans-stilbene oxide (TSO) on hepatic and renal xenobiotic metabolizing-enzyme activities and bromobenzene-induced toxicity was quantified in adult male Sprague-Dawley rats. Rats were fed a regular diet or the same diet supplemented with 2.5 g TSO/kg diet for 10 days. TSO treatment did not alter hepatic or renal arylhydrocarbon hydroxylase activity, but significantly increased glutathione S-transferase and uridine diphosphoglucuronyl transferase activities in both organs. In addition, TSO increased hepatic, but not renal, epoxide hydrolase activity. The same treatment did not produce adverse effects on renal or hepatic functions, but markedly potentiated bromobenzene hepatotoxicity. A single dose of bromobenzene (0.2 ml/kg) caused a slight increase in serum glutamic pyruvic transaminase (SGPT) activity and minor hepatic necrosis in animals fed the control diet; the same dose of bromobenzene markedly increased SGPT activity and produced severe hepatic necrosis in the TSO-fed animals.