Evaluation of the Structure and Biological Activities of Condensed Tannins from Acanthus ilicifolius Linn and Their Effect on Fresh-Cut Fuji Apples.

Condensed tannins (CT_S) have been isolated and purified from leaves of Acanthus ilicifolius Linn. And their structures were investigated by three methods: ¹³C nuclear magnetic resonance (¹³C NMR), reversed-phase high-performance liquid chromatography (RP-HPLC), and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The results showed that the CT_S were a mixture of catechin/epicatechin, galatechin/epicatechin, and amphicin/epigalin, and that the polymer chain lengths were 3-mers to 14-mers. Antityrosinase activities and antioxidant activities of the CT_S from A. ilicifolius leaves were further studied. The IC₅₀ of the CT_S on mushroom tyrosinase activity was determined to be 19.7 ± 0.13 μg/mL, and inhibition type analyses indicated that the CT_S were mixed type inhibitors and their inhibition CT_S was reversible. The CT_S from A. ilicifolius leaves also exhibited potential antioxidant activity. The IC₅₀ of DPPH and ABTS scavenging activities were 104 ± 0.894 μg/mL and 86 ± 0.616 μg/mL, respectively. And the FRAP value was 758.28 ± 2.42 mg AAE/g. In addition, we found that the CT_S from A. ilicifolius leaves had an excellent effect on preserving the quality of fresh-cut apples by preventing apples from browning through reducing polyphenol oxidase activities in apples.