[Isolation and preparation of flavones from the leaves of Lindera aggregata using high speed counter-current chromatography].

Lindera aggregata (Sims) Kosterm. belongs to Lauraceae family and is distributed in the southern parts of China, Japan and southeastern Asia. The leaves of this plant are frequently used in folk medicine, as antioxidant, antiinflammatory and antibacterial. In previous reports, eleven flavones were isolated from this plant. The study on the flavone constituents of the leaves of this plant led to the isolation of five flavone compounds, as quercetin-3-O-beta-D-glucoside (1), quercetin-5-O-beta-D-glucoside (2), quercetin-3-O-beta-D-arabinfuranoside (3), quercetin-3-O-rhamnopyranoside (4) and kaempferol-7-O-alpha-L-rhamnopyranoside (5), by means of high speed counter-current chromatographic (HSCCC) procedures. A two-phase solvent system composed of hexane-ethyl acetate-n-butanol-acetic acid-water (2: 4: 2: 1.5: 6, v/v) was used at a flow rate of 2.0 mL/min, while the apparatus rotated at 800 r/min and the effluent was detected at 280 nm. Compounds 1, 2, 3 and 5 were for the first time obtained from Lindera aggregata (Sims) Kosterm.