Biochimica et Biophysica Acta - General Subjects 1979-May
Studies on proteinases from Calotropis gigantea latex. I. Purification and some properties of two proteinases containing carbohydrate.
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Two proteinase containing carbohydrate, called calotropain-FI and calotropain-FII, were purified from Calotropis gigantea latex by CM-Sephadex C-50 chromatography. Both calotropain-FI and FII were found to be homogeneous by rechromatography on CM-Sephadex C-50, gel filtration on Sephadex G-100, electrophoresis on polyacrylamide gel and by N-terminal amino acid analysis. Some properties of these enzymes are reported.