Jasmonic acid and hydrogen sulfide modulate transcriptional and enzymatic changes of plasma membrane NADPH oxidases (NOXs) and decrease oxidative damage in Oryza sativa L. during thiocyanate exposure

It is evident that the plasma membrane NADPH oxidases (NOXs) play an important role in the generation of superoxide radicals (O₂^-•) in plants during defense responses. This study was to clarify activation of NOXs in oxidative damage in Oryza sativa during SCN^- exposure, particularly in the roles of jasmonic acid (JA) and hydrogen sulfide (H₂S) on transcriptional and enzymatic changes of NOXs. Results indicated that enzymatic activity of NOXs in both roots and shoots was significantly enhanced during SCN^- exposure, whereas the application of JA and H₂S donor (NaHS) significantly repressed NOXs activity in SCN-treated rice seedlings. Similarly, ROS analysis showed that SCN^- exposure elevated the content of O₂^-•, hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) in rice tissues significantly, whereas decreases in O₂^-• and H₂O₂ were detected in roots and shoots of SCN-treated rice seedlings due to application of JA and NaHS. PCR analysis revealed different expression patterns of 7 plasma membrane-localized NOX genes in rice roots and shoots against SCN^- exposure, suggesting that various isogenes of NOXs might regulate and determine activity of NOXs in rice organs. In conclusion, SCN^- exposure was able to trigger activation of NOXs effectively, and led to oxidative damage and lipid peroxidation; the effects of JA and NaHS on inactivation of NOXs was evident and tissue specific, which in turn modulated ROS accumulation in rice plants.

Keywords: Hydrogen sulfide; Jasmonic acid; NADPH oxidases; ROS; Rice seedlings; Thiocyanate.