Journal of the Science of Food and Agriculture 2020-Feb
Quantifying non-fibrous carbohydrates, acid detergent fiber and cellulose of forage through an in vitro gas production technique.
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RESULTS
Linear models were generated and validated as alternatives of quantifying neutral detergent-soluble fiber (NDSF), Starch (St)/hemicellulose (Hem), and cellulose (Cel) through GP. Residuals of models obtained from peaks of GP [0-8 h (GP-8), >8-24 h (GP-24), >24-48 h (GP-48), >24-81 h (GP-81)]) of 0.02, 0.04, 0.08, 0.12, 0.20 g of glucose (Glu), St and Cel incubations were analyzed in mixtures of Glu, Starch and Cel (0.05, 0.10, 0.15 g; 27 combinations). The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals (SR)<0.09. Analysis with Leucaena (Leucaena leucocephala Lam. de Wit) and star grass (Cynodon nlemfuensis Vanderyst) consider high-protein ingredients.