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Simple procedure for preparation of bluetongue virus and epizootic hemorrhagic disease virus antigens for agar gel immunodiffusion.
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A simplified procedure was developed for preparing soluble antigen from two related orbiviruses, bluetongue and epizootic hemorrhagic disease viruses, for agar gel immunodiffusion. The antigens gave excellent results in both micro-agar gel diffusion (agar gel precipitin) and macro-agar gel diffusion
Comparison of sorbitol MacConkey and hemorrhagic coli agars for recovery of Escherichia coli O157:H7 from brie, ice cream, and whole milk.
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The relative efficacies of hemorrhagic coli (HC) agar and several formulations of sorbitol MacConkey (SorMac) agar, with and without 0.1% (w/v) 4-methyllumbelliferyl-beta-D-glucuronide (MUG), in recovering unstressed and heat-stressed Escherichia coli O157:H7 from Brie cheese, ice cream, and whole
Agar gel diffusion precipitin test for hemorrhagic enteritis of turkeys.
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First Report of Stem Bleeding in Coconut Caused by Ceratocystis paradoxa in Hainan, China.
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Stem bleeding of coconut was discovered in 2009 in Hainan, China. Affected trunk areas exhibited dark discoloration and a reddish brown or rust-colored liquid bleeding from different points. Stem tissues under the lesions rotted and became brownish yellow to black. Affected plants died within 3 to 4
Experimental bluetongue and epizootic hemorrhagic disease virus infection in California black-tailed deer.
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Four adult black-tailed deer (Odocoileus hemioneus columbianus) and five fawns were inoculated with bluetongue virus (BTV) and one adult deer was inoculated with epizootic hemorrhagic disease (EHD) virus to produce clinical signs and lesions of hemorrhagic disease. Serologic response was monitored
Epizootic hemorrhagic disease virus and bluetongue virus serotype distribution in white-tailed deer in Georgia.
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Serum samples collected from 1,396 white-tailed deer (Odocoileus virginianus) in five areas of Georgia (USA) from 1989 to 1991 were tested for precipitating and serum neutralizing (SN) antibodies to the enzootic North American epizootic hemorrhagic disease virus (EHDV) and bluetongue virus (BTV)
Precipitating antibodies to epizootic hemorrhagic disease and bluetongue viruses in white-tailed deer in the southeastern United States.
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From 1981 to 1989, sera were collected from 3,077 white-tailed deer (Odocoileus virginianus) in Georgia and from 1,749 deer from 12 additional states in the southeastern United States. In Georgia, prevalence of precipitating antibodies to epizootic hemorrhagic disease virus (EHDV) and bluetongue
A comparison of four methods for the detection of turkey haemorrhagic enteritis virus and antigen in experimentally infected turkeys.
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Four tests (agar gel immunodiffusion, electron microscopy, direct immunoperoxidase staining and direct immunofluorescence) were compared for the detection of turkey haemorrhagic enteritis virus or antigen in spleen and faeces samples. Direct immunoperoxidase and immunofluorescent staining of spleen
Detergent-treated Newcastle disease virus as an agar gel precipitin test antigen.
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A soluble Newcastle disease virus (NDV) agar gel precipitin (AGP) antigen was prepared by treating 100-fold concentrated NDV with a nonionic detergent. Virus concentration prior to detergent treatment was best accomplished by ultracentrifugation or by a simple, less expensive, and more practical
Equine infectious anemia: sensitivity of the agar-gel immunodiffusion test, and the direct and the indirect complement-fixation tests for the detection of antibodies in equine serum.
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The comparative values of the direct, the indirect complement-fixation and the agar-gel immunodiffusion tests were assessed for the diagnosis of equine infectious anemia. Antibodies were detected on the agar-gel immunodiffusion test as early as 18 days post-inoculation in the serums of
The use of sorbitol-MacConkey agar in conjunction with a specific antiserum for the detection of Vero cytotoxin-producing strains of Escherichia coli O 157.
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Using DNA probes specific for the genes encoding Vero cytotoxins 1 and 2 in hybridization experiments on faecal samples, Vero cytotoxin-producing Escherichia coli (VTEC) of serogroup O 157 were detected in 21 of 63 cases of haemorrhagic colitis, 9 of 31 cases of non-bloody diarrhoea and 14 of 68
Comparison of sorbitol MacConkey agar and a two-step method which utilizes enzyme-linked immunosorbent assay toxin testing and a chromogenic agar to detect and isolate enterohemorrhagic Escherichia coli.
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Enterohemorrhagic Escherichia coli (EHEC) and specifically serotype O157:H7 are a significant cause of hemorrhagic gastrointestinal disease and the hemolytic uremic syndrome. Methods currently used in clinical microbiology labs, such as sorbitol-MacConkey (SMAC) agar, reliably detect only O157:H7.
Crimean hemorrhagic fever-Congo (CHF-C) virus antibodies in man, and in domestic and small mammals, in Iran.
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A Crimean hemorrhagic fever-Congo (CHF-C) virus antibody survey was carried out in Iran with sera from man and several animal species; this survey was done by means of agar gel diffusion precipitation (AGDP) test with the following results (percent positive of number tested): men, 13% of 351; sheep,
Effect of pressure distribution of shockwave on renal hemorrhage after extracorporeal shockwave lithotripsy: comparison of EDAP LT-01 and Siemens Lithostar.
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BACKGROUND
Renal hemorrhage is the most common adverse effect of SWL, and it has been speculated to be related to the type of lithotripter used.
METHODS
We investigated the incidence of renal hemorrhage in patients with urinary stones who underwent lithotripsy using either the EDAP LT-01 or the
Further studies on in vitro and in vivo assays of hemorrhagic enteritis virus (HEV).
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Isolation of hemorrhagic enteritis virus (HEV) from spleens of infected turkeys in the MDTC-RP19 lymphoblastoid cell line was compared with detection of HEV antigen in the same spleens using the agar gel precipitation (AGP) test. A concordance of 80% was found between the two assays. Virus isolation
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