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6 resultater
Study of mRNA expression of Arabidopsis thaliana under stimulation using modified differential display RT-PCR with silver staining.
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We studied differential expressions of Arabidopsis thaliana seedlings under sound stimulation using modified differential display RT-PCR with silver staining in this paper. Six differentially expressed cDNA fragments were obtained. Molecular weight of fragments was between 200 and 600 bp,
Whole-mount silver staining of Arabidopsis and maize: a new method for staining secondary wall thickenings in tracheary elements.
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Secondary wall thickenings in tracheary elements were specifically stained by incubation of Arabidopsis and maize in Silver Stain Plus (Bio-Rad) staining solution, after pretreatment with SDS and ethanol solution. Scanning electron microscopic analysis of sections of celery revealed that silver
Analysis of the synaptonemal complex in Brassica using TEM.
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Much of meiosis research is focussed on Arabidopsis thaliana, largely due to the significant advantages it brings, having a small sequenced genome with comparatively little repetitive DNA, the ease of forward and reverse genetics, and a short life cycle. On the other hand, due the small genome size
Properties of serine:glyoxylate aminotransferase purified from Arabidopsis thaliana leaves.
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The photorespiratory enzyme L-serine:glyoxylate aminotransferase (SGAT; EC 2.6.1.45) was purified from Arabidopsis thaliana leaves. The final enzyme was approximately 80% pure as revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with silver staining. The identity of the enzyme
Comparative proteomic analysis of Arabidopsis mature pollen and germinated pollen.
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Proteomic analysis was applied to generating the map of Arabidopsis mature pollen proteins and analyzing the differentially expressed proteins that are potentially involved in the regulation of Arabidopsis pollen germination. By applying 2-D electrophoresis and silver staining, we resolved 499 and
A nuclear-encoded subunit of the photosystem II reaction center.
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A nuclear-encoded polypeptide of 6.1 kDa was identified in isolated photosystem II (PSII) reaction center from Spinacia oleracea. The hydrophobic membrane protein easily escapes staining procedures such as Coomassie R-250 or silver staining, but it is clearly detected by immunodecoration with
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