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Plasma and urinary amino acid pattern in severe burn patients-evolution throughout the healing period.
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Plasma and urinary amino acids were measured in 12 severely burned adults (20% of body surface area or more). Measurements were made on the day of injury, and seven times thereafter until the 28th day after trauma. During the first 7 days, a decrease in plasma concentrations of some glucose
Ornithine alpha-ketoglutarate metabolism after enteral administration in burn patients: bolus compared with continuous infusion.
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Ornithine alpha-ketoglutarate (OKG) has been successfully used as an enteral supplement in the treatment of catabolic states, including burn injury. However, specific questions remain unanswered concerning burn patients, including OKG metabolism and metabolite production, appropriate mode of
Prolyl hydroxylase activity in human normal skins and post-burn scars.
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Post-burn granulation tissues showed an extraordinary high prolyl hydroxylase (EC 1.14.11.2; proline, 2-oxoglutarate dioxygenase) activity, 25 to 50 times higher than the mean value of human normal skins from the frontal thighs of 15 subjects, 385 +/- 247 (S.D.) cpm/min/g of wet weight tissue. The
Proline metabolism in adult male burned patients and healthy control subjects.
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Postabsorptive proline flux, oxidation, and endogenous biosynthesis were determined in five severely burned intensive-care-unit patients (mean age 27 y) and in six healthy, young-adult control subjects. Continuous primed, intravenous, 160-min, dual stable-isotope-tracer infusions of L-[1-13C]proline
Serum and erythrocyte amino acid pattern: studies on major burn cases.
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Venous serum amino acids were measured in 13 patients with major burns. Erythrocyte amino acids and plasma cortisol, blood sugar and urine catecholamine were measured in two representative subgroups respectively. After burn injury, serum proline, glycine, valine, isoleucine and arginine were
[Scaffolds based on collagen and chitosan for post-burn tissue engineering].
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Porous scaffolds based on collagen and chitosan have been obtained from mixed bio-polymeric solutions and mixture freeze-drying method in the purpose of using them as materials for post-burns tissue regeneration.
METHODS
Soluble collagen from bovine leather was obtained by acid-base extraction
Glutamine metabolism and function in relation to proline synthesis and the safety of glutamine and proline supplementation.
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At normal intakes, dietary glutamine and glutamate are metabolized by the small intestine and essentially all glutamine within the body is synthesized de novo through the action of glutamine synthetase. The major sites of net glutamine synthesis are skeletal muscle, lung, and adipose tissue and,
Effect of enterally administered ornithine alpha-ketoglutarate on plasma and urinary amino acid levels after burn injury.
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Eight severely burned patients received 10 grams twice a day of enterally administered ornithine alpha-ketoglutarate. Variations in their plasma and urinary amino acid levels were compared with those from six severely burned control patients. The study covered the period from the fourth day to the
Synergy of nitric oxide and silver sulfadiazine against gram-negative, gram-positive, and antibiotic-resistant pathogens.
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The synergistic activity between nitric oxide (NO) released from diazeniumdiolate-modified proline (PROLI/NO) and silver(I) sulfadiazine (AgSD) was evaluated against Escherichia coli, Enterococcus faecalis, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus
[Arginine--metabolism and functions in the human organism].
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L-arginine plays important roles in the metabolism of an organism. It is the precursor for the synthesis of proteins and other molecules of great biological importance, including nitric oxide, ornithine, polyamines, agmatine, proline, glutamate, creatine, dimethylarginine, and urea. For young
Effects of caffeic acid phenethyl ester and epidermal growth factor on the development of caustic esophageal stricture in rats.
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OBJECTIVE
An experimental study was performed to modify the healing response in caustic esophageal burns to prevent stricture development. Two different agents with different modes of actions, caffeic acid phenethyl ester (CAPE) and epidermal growth factor (EGF), were studied. CAPE has
Inhibitory effects of lanthanum chloride on extracellular matrix in injury tissues of rats.
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The propose of the present study is to investigate the effects of lanthanum chloride (LaCl(3)), a rare earth compound, on extracellular matrix molecules in rat wound tissues, hoping to provide a clue for further study in reducing excessive extracellular matrix formation in trauma and burn, as well
Effects of intermittent hyperbaric oxygen on guinea pig lung elastin and collagen.
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The effect of high oxygen pressure on collagen and elastin turnover in lung parenchyma tissue was studied in guinea pigs. Three groups of animals was used. One group (I) was given a clinically excessive exposure of 3 h daily for 9 days of 3 ATA oxygen. A second group (II) was given 3 ATA compressed
Regulation of extracellular matrix proteins by transforming growth factor beta1 in cultured pulmonary endothelial cells.
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Transforming growth factor beta-1 (TGF-beta1), which is present in lung tissue, has been suggested to play a role in modulating vascular cell function in vivo. The action of TGF-beta1 in vivo, especially at the local site of application to connective tissue, is anabolic and leads to pulmonary
Gas chromatography-mass spectrometry determination of 18O2 in 18O-labelled 4-hydroxyproline for measurement of collagen synthesis and intracellular degradation.
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The use of gas chromatography-mass spectrometry (GC-MS) and 18O2, a stable isotope which is incorporated into collagen during the post-translational conversion of proline to hydroxyproline, offers the potential advantages of high levels of sensitivity and specificity as compared to other techniques
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