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Localization of sucrose synthase in wheat roots: increased in situ activity of sucrose synthase correlates with cell wall thickening by cellulose deposition under hypoxia.
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Sucrose synthase (SuSy; EC 2.4.1.13) plays a prominent role in O(2) deficiency and functions at a branch point, partitioning sucrose between cell wall biosynthesis and glycolysis. The cleavage of sucrose by SuSy was localized in wheat ( Triticum aestivum L. cv. Alcedo) roots subjected to 4 days of
Biocompatibility of cuprophan and cellulose acetate membranes. Prevention of dialysis hypoxemia and leucopenia by ticlopidine.
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The study is a comparison of 4 successive hemodialysis (HD) sessions on each patient, 2 with cuprophan (CU) membrane (Gambro 120 M) and 2 with Cellulose acetate (CA) (Cordis Dow 3500). 60 minutes prior to the HD session placebo or Ticlopidine (500 mg) was administered orally to each patient.
Composition analysis and anti-hypoxia activity of polysaccharide from Brassica rapa L.
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Crude water-soluble polysaccharides (BRP) were extracted from the root of Brassica rapa L. using boiling-water. The polysaccharides were successively purified by chromatography on DEAE-cellulose and Sephadex G-100 column, giving three major polysaccharide fractions termed BRP1-1, BRP2-1, BRP3-1. The
Linking cellulose fiber sediment methyl mercury levels to organic matter decay and major element composition.
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Methylation of mercury (Hg) to highly toxic methyl Hg (MeHg), a process known to occur when organic matter (OM) decomposition leads to anoxia, is considered a worldwide threat to aquatic ecosystems and human health. We measured temporal and spatial variations in sediment MeHg, total Hg (THg), and
Fatal foreign-body granulomatous pulmonary embolization due to microcrystalline cellulose in a patient receiving total parenteral nutrition: all crystals are not what they seem.
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Pulmonary foreign-body granulomatous embolization has been described secondary to crystal precipitation in total parenteral nutrition (TPN) as well as when pharmaceutical tablets are crushed and injected intravenously. Extensive granulomatous embolization may cause pulmonary hypertension and death
Metabolism of ATP during anoxia in guinea pig seminal vesicle mucosa.
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The content of adenine nucleotides in guinea pig seminal vesicles was determined enzymatically. Inosine monophosphate (IMP) was isolated from other tissue nucleotides by two-dimensional thin-layer chromatography on cellulose PEI plates, eluted, and quantitated spectrophotometrically. The
[Qualitative and quantitative assessment of blood erythrocytes of intact animals under the effect of 5-fluorouracil in solution and in microspheres made of acetylcellulose combined with short-term gas hypoxia].
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Resistance of erythrocyte membranes, estimated by means of ultrasound hemolysis, was altered 7 days before the cells quantitative changes after simultaneous treatment of intact animals with 5-fluorouracil, administered as solution or in acetate cellulose drug-containing granules, and short-term gas
Hypoxia in fibroblast cultures. 4. Cell density, glucose utilization and acid mucopolysaccharides in 1% O2 concentration.
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Secondary cultures of embryonic rat fibroblasts incubated in Eagle medium with Hepes buffer at pH 6.6 and 7.4 were maintained in hypoxia of 1% O2 for 48 hours. The acid mucopolysaccharides were isolated from medium plus cells and fractionated on cellulose microcolumns according to SVEJCAR and
Grape seed extract effects in brain after hypobaric hypoxia.
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Hypoxia induces a wide range of deleterious effects at the cellular level due to an increased production of reactive oxygen species (ROS). Polyphenols from grape seeds, which are potent antioxidants might protect the brain against oxidative stress produced by hypobaric hypoxia. The brain effects of
Effects of the neutral endopeptidase inhibitor, SCH 42495, on the cardiovascular remodelling secondary to chronic hypoxia in rats.
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1. We have investigated the effects of inhibition of neutral endopeptidase on the cardiovascular remodelling secondary to chronic hypoxia in rats using a novel neutral endopeptidase inhibitor, SCH 42495. 2. Rats were divided into four groups, two of which were maintained in a normobaric, hypoxic
Neutral endopeptidase (NEP) inhibition in rats with established pulmonary hypertension secondary to chronic hypoxia.
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1. Atrial natriuretic peptide (ANP) causes vasorelaxation in the pulmonary vasculature. ANP levels are elevated in conditions characterized by pulmonary hypertension and it has been hypothesized that ANP may be autoregulatory in the pulmonary circulation. 2. One route of ANP metabolism in vivo is by
Hypoxia differentially enhances the effects of transforming growth factor-beta isoforms on the synthesis and secretion of glycosaminoglycans by human lung fibroblasts.
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Interstitial lung diseases associated with hypoxia, such as lung fibrosis, are characterized by enhanced production of transforming growth factor-beta (TGF-beta) and increased deposition of extracellular matrix (ECM) molecules, including glycosaminoglycans (GAGs). In this study, we investigated the
The increase in fetal hemoglobin synthesis in the fetal lamb during hyperglycemic hypoxemia.
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To determine whether fetal hypoxemia induced by hyperglycemia has any effect on the proportions of fetal and adult hemoglobin synthesized during fetal development, hemoglobin synthesis was determined after a period of hyperglycemic hypoxemia in the fetal lamb. These experiments were carried out at a
Effects of anoxia on protein phosphatase in turtle organs: purification and properties of protein phosphatase type-1 from turtle liver.
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Protein phosphatase type 1 (PP-1) was analyzed in organs of the red-eared slider turtle, Trachemys scripta elegans, a species capable of long-term anoxia survival. During anoxic submergence at 7 degrees C, PP-1 activity in liver rapidly decreased to 63% of the control value within the first hour and
Synergistic role of three dimensional niche and hypoxia on conservation of cancer stem cell phenotype.
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Hypoxia is a pathalogical condition in which tissues are deprived of adequate oxygen supply. The hypoxia effect on tumors has a critically important role on maintenance of cancer stem cell phenotype. The aim of this study is to investigate the effects of hypoxia on cancer stem cells on three
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