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glucuronic acid/atrofi
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[STUDIES ON THE BEHAVIOR OF GLUCURONIC ACID IN HEPATOLENTICULAR DEGENERATION].
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Exploiting the carboxylate chemical shift to resolve degenerate resonances in spectra of 13C-labelled glycosaminoglycans.
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Glycosaminoglycans (GAG) are important vertebrate extracellular matrix polysaccharides that comprise repeated units of an acidic and an N-acetylated sugar. The constituent acidic sugars are central to their biological functions, but have been largely inaccessible to NMR because the (1)H resonances
Glucuronic acid-containing glycosaminoglycans occur in otoconia: cytochemical evidence by hyaluronidase-gold labeling.
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Localization of glucuronic acid-containing glycosaminoglycans in the gerbil utricle was examined, using a hyaluronidase-gold labeling technique with special emphasis on the otoconia. Otoconia and the gelatinous layer of the otoconial membrane were strongly labeled by hyaluronidase-gold. The
[Preclinical toxicological study of D-glucuronic acid].
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A new D-glucuronic acid (DGA) preparation was studied for toxicological safety on a preclinical level. The results obtained upon a single acute DGA administration in rats, mice, and rabbis showed that the drug exhibits moderate toxicity. A one-month treatment of rats (at a single daily dose of 50,
The degradation of glycosaminoglycans by intestinal microflora deteriorates colitis in mice.
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The biosynthesis and modification of mucopolysaccharides and glycosaminoglycans (GAGs), secreted from gastrointestinal mucosal cells, are increased in colitis and influence the viability of the defense barrier. Therefore, to evaluate the role of GAG-degrading intestinal microflora during the
Comparative histochemical and biochemical analysis of endogenous receptors for glycoproteins in human and pig peripheral nerve.
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Endogenous sugar-binding proteins were localized in sections of human and pig peripheral nerves by the application of two types of labelled ligands: neoglycoproteins (chemically glycosylated carrier proteins that had proven to be histochemically inert) and desialylated, naturally occurring
Expression and regulation of UDP-glucuronate: neolactotetraosylceramide glucuronyltransferase in the nervous system.
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Sulfoglucuronyl glycolipids (SGGLs) are temporally and spatially regulated molecules present in the nervous system during its development. The characteristics of the rat brain enzyme glucuronyltransferase involved in the biosynthesis of SGGLs have been described. The enzyme catalyzes the transfer of
Purification, characterization and functional cloning of inositol oxygenase from Cryptococcus.
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The enzyme inositol oxygenase (myo-inositol : oxygen oxidoreductase; E.C. 1.13.99.1) is a monooxygenase that converts inositol into glucuronic acid in the presence of molecular oxygen. This enzyme is integrated into a pathway leading to either degradation and energy production or the biosynthesis of
[Preliminary proteomics analysis of the total proteins of HL Type cytoplasmic male sterility rice anther].
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The proteins of HL type cytoplasmic male sterility rice anther of YTA (CMS) and YTB (maintenance line) were separated by two-dimensional electrophoresis with immobilized ph (3-10 non-linear) gradients as the first dimension and SDS-PAGE as the second. The silver-stained proteins spots were analyzed
UDP-glucuronyltransferase in perfused rat liver and in microsomes. Effects of CCl4 injury.
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To elucidate the disparity between glucuronidation rates in vivo and UDP-glucuronyltransferase in vitro after CCl4 injury, the time course of the effects of CCl4 (0.25 ml/kg) on kinetic properties of UDP-glucuronyltransferase (l-naphthol as substrate) was examined in rat liver homogenates and
An experimental study on reconstruction of the condyle of the temporomandibular joint using free autogenous costal perichondrial grafts in rabbits.
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OBJECTIVE
The purpose of this study was to develop a new method to regenerate articular cartilage of the temporomandibular joint (TMJ) by transplantation of free autogenous costal perichondrium (PC).
METHODS
In the study, 50 adult rabbits received the operation. For the surgery in the test group,
Head-column field-amplified sample stacking in presence of siphoning. Application to capillary electrophoresis-electrospray ionization mass spectrometry of opioids in urine.
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Capillary electrophoresis (CE) with head-column field-amplified sample stacking (FASS) in presence of a water plug inserted at the capillary tip is a robust approach providing a more than 1000-fold sensitivity enhancement when applied to low-conductivity samples that are analyzed in an integrated
Metabolism of L-ascorbic acid in rats under in vivo administration of mercury: effect of L-ascorbic acid supplementation.
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1. Rats toxicated with mercury showed drastic fall in growth rate and supplementation of L-ascorbic acid to these rats could not reverse this effect. The contents of L-ascorbic acid and of D-glucuronic acid in the urine of the toxicated animals were decreased which could be counteracted by
Cloning and expression of a novel galactoside beta1, 3-glucuronyltransferase involved in the biosynthesis of HNK-1 epitope.
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We isolated a cDNA encoding a novel glucuronyltransferase, designated GlcAT-D, involved in the biosynthesis of the HNK-1 carbohydrate epitope from rat embryo cDNA by the degenerate polymerase chain reaction method. The new cDNA sequence revealed an open reading frame coding for a protein of 324
Effect of glucocorticoids on glycosaminoglycan metabolism in cultured human skin fibroblasts.
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Human skin fibroblasts were exposed to 3 anti-inflammatory steroids in order to study their effects on the glycosaminoglycan metabolism. The potent glucocorticoids, fluocinolone acetonide and budesonide, even at low concentrations strongly reduced the accumulation of hyaluronic acid and sulfated
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