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glycoalkaloid/kartoffelplante
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Simple and sensitive method for determination of glycoalkaloids in potato tubers by high-performance liquid chromatography with chemiluminescence detection.
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A novel, simple and sensitive high-performance liquid chromatographic method for the determination of the potato glycoalkaloids, alpha-solanine and alpha-chaconine, based on the chemiluminescent reaction of tris(2,2'-bipyridine)ruthenium(III) has been developed. The calibration graph was linear in
[Study of the interaction of main potato glycoalkaloids in inhibition of immobilized butyryl cholinesterase].
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The interaction of main potato glycoalkaloids alpha-solanine and alpha-chaconine in inhibition of horse serum butyryl cholinesterases immobilized on the pH-sensitive field-effect transistors has been investigated. The method of isobol diagram of Loewe and Muishnek has been used for interpretation of
Liquid chromatographic determination of the glycoalkaloids alpha-solanine and alpha-chaconine in potato tubers: NMKL Interlaboratory Study. Nordic Committee on Food Analysis.
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Twelve laboratories participated in a collaborative study to evaluate precision parameters of a liquid chromatographic method for analysis of the glycoalkaloids alpha-solanine and alpha-chaconine in potato tubers. Samples consisted of frozen potato tuber homogenates distributed as 3 blind duplicates
Hepatic ornithine decarboxylase induction by potato glycoalkaloids in rats.
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The induction of hepatic ornithine decarboxylase (ODC) activity in rat livers by the potato glycoalkaloids alpha-solanine, alpha-chaconine, and their aglycone solanidine, has been studied. Ip administration of alpha-solanine at 7.5, 15 and 30 mg/kg body weight produced markedly elevated enzyme
A capillary electrophoresis laser-induced fluorescence method for analysis of potato glycoalkaloids based on a solution-phase immunoassay. 2. Performance evaluation.
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Glycoalkaloids (GAs) occur naturally in potatoes and are toxic to humans and animals. The objective of the present study was to evaluate the performance of a solution-phase immunoassay coupled to capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection for the determination of
Determination of potato glycoalkaloids and their aglycone in blood serum by high-performance liquid chromatography. Application to pharmacokinetic studies in humans.
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The development of a high-performance liquid chromatography (HPLC) method for the separation and quantification of potato glycoalkaloids and their aglycone solanidine in blood serum is reported. High selectivity was obtained by using solid-phase extraction followed by off-line dual-column HPLC.
Light Regulation of Chlorophyll and Glycoalkaloid Biosynthesis During Tuber Greening of Potato S. tuberosum
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Potato, S. tuberosum, is one of the most important global crops, but has high levels of waste due to tuber greening under light, which is associated with the accumulation of neurotoxic glycoalkaloids. However, unlike the situation in de-etiolating seedlings, the mechanisms underlying tuber
Colorado potato beetle toxins revisited: evidence the beetle does not sequester host plant glycoalkaloids.
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The Colorado potato beetle feeds only on glycoalkaloid-laden solanaceous plants, appears to be toxic to predators, and has aposematic coloration, suggesting the beetle may sequester alkaloids from its host plants. This study tested 4th instars and adults, as well as isolated hemolymph and excrement,
Cardioinhibitory properties of potato glycoalkaloids in beetles.
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The semi-isolated heart bioassay was used to evaluate the effect of glycoalkaloids extracted from potato leaves on the heart contractile activity of three beetle species Zophobas atratus, Tenebrio molitor and Leptinotarsa decemlineata. The dose-response curves indicated species specific action of
Determination of potato glycoalkaloids using a liposome immunomigration, liquid-phase competition immunoassay.
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Polyclonal anti-solanine antibodies were raised and used in the assembly of a liposome immunomigration, liquid-phase competition strip immunoassay. In this format, a similar cross-reactivity was observed between the glycoalkaloids alpha-solanine and alpha-chaconine. The strip assay was implemented
Analysis of potato glycoalkaloids by gas-liquid chromatography of alkaloid components.
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A gas-liquid chromatographic (GLC) method has been developed for the analysis of potato glycoalkaloids by direct determination of the alkaloid components isolated by hydrolysis with 1N ethanolic HCl. Recoveries of solanidine from potato tubers and foliage fortified with solanine over a range
Glycoalkaloid and calystegine levels in table potato cultivars subjected to wounding, light, and heat treatments.
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Potato tubers naturally contain a number of defense substances, some of which are of major concern for food safety. Among these substances are the glycoalkaloids and calystegines. We have here analyzed levels of glycoalkaloids (α-chaconine and α-solanine) and calystegines (A₃, B₂, and B₄) in potato
Evaluation of glycoalkaloids in tubers of genetically modified virus Y-resistant potato plants (var. Désirée) by non-aqueous capillary electrophoresis coupled with electrospray ionization mass spectrometry (NACE-ESI-MS).
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The glycoalkaloid content of transgenic potatoes was evaluated by an optimised method based on non-aqueous capillary electrophoresis coupled on-line with electrospray ionization-mass spectrometry (NACE-ESI-MS). The potato material consisted of tubers from a conventional cv. Désirée and from three
Inhibition of steroid glycoalkaloid accumulation by arachidonic and eicosapentaenoic acids in potato.
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Eicosapentaenoic and arachidonic acids extracted from the fungus Phytophthora infestans elicit the accumulation of fungitoxic sesquiterpenoid stress metabolites and inhibit the accumulation of steroid glycoalkaloids in potato tubers. This dual activity, which did not occur with other saturated and
Degradation of the potato glycoalkaloids--alpha-solanine and alpha-chaconine in groundwater.
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The potato glycoalkaloids alpha-chaconine and alpha-solanine are produced in high amounts in potato plants from where release to soil takes place. Degradation of the compounds in groundwater was investigated, as their fate in the terrestrial environment is unknown. Abiotic and microbial degradation
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