After the run-in period, volunteers will receive casein supplementation (15 % of energy intake) for 3 weeks.

After the run-in period, volunteers will receive soy protein supplementation (15 % of energy intake) for 3 weeks.

After the run-in period, volunteers will receive maltodextrin supplementation (15 % of energy intake) for 3 weeks.

Overweight

Healthy

casein supplementation

soy protein supplementation

maltodextrin supplementation

Effects of High Protein Diet on the Large Intestine in Overweight Humans

Gene expression in biopsies collected before (baseline) and after 3 week dietary intervention will be measured by transcriptomic analyses. Differential gene expression will be expressed as relative units (fold change).

Microbiota composition will be analysed in stool samples before (baseline) and after 3 week dietary intervention using metagenomic analyses and will be expressed as bacterial relative abundance.

Fecal water metabolites will be analysed in stool samples before (baseline) and after 3 week dietary intervention using RMN analyses and will be expressed as metabolites relative abundance.

hydrogen sulfide/fedme

Effects of High Protein Diet on the Large Intestine in Overweight Humans

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