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keratitis/phosphatase
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[Alkaline and acid phosphatase activity and localization in the eye tissues and fluids in herpetic keratitis].
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Bacterial and fungal keratitis in Upper Egypt: in vitro screening of enzymes, toxins and antifungal activity.
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OBJECTIVE
This work was conducted to study the ability of bacterial and fungal isolates from keratitis cases in Upper Egypt to produce enzymes, toxins, and to test the isolated fungal species sensitivity to some therapeutic agents.
METHODS
One hundred and fifteen patients clinically diagnosed to
Role of miR-155 in the pathogenesis of herpetic stromal keratitis.
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Ocular infection with herpes simplex virus 1 can result in a chronic immunoinflammatory stromal keratitis (SK) lesion that is a significant cause of human blindness. A key to controlling SK lesion severity is to identify cellular and molecular events responsible for tissue damage and to manipulate
Studies on a strain of Fusarium solani (Mart.) Sacc. Isolated from a case of mycotic keratitis.
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A strain of Fusarium solani (Mart.) Sacc. (IMI-216517), isolated from a patient of mycotic keratitis, produced experimental keratomycosis in albino rabbit cornea and survived in internal tissues of albino mice for varying periods. Alantolactone, isolated from the plant - Inula racemosa Hook. f.
Box Behnken design of siRNA-loaded liposomes for the treatment of a murine model of ocular keratitis caused by Acanthamoeba.
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Acanthamoeba keratitis is an ophthalmic disease with no specific treatment that specially affects contact lens users. The silencing of serine phosphatase (SP) and glycogen phosphorylase (GP) proteins produced by Acanthamoeba has been shown to significantly reduce the cytopathic effect, although no
Enzyme activities in the rabbit cornea during immunogenic keratitis.
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In rabbits inflammation of the cornea was induced by intrastromal injection of horse serum. Between 2 and 4 weeks after injection, infiltration of the cornea with leukocytes and neovascularization could be observed. During this period, the rabbits were killed and their corneas analyzed for protein,
[The relationship between corneal lymphangiogenesis and inflammation index after corneal alkali injury].
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OBJECTIVE
To discuss the relationship between corneal lymphangiogenesis and inflammation index (IF) in alkali burned corneas.
METHODS
Experimental research. Rat corneal hemangiogenesis and lymphangiogenesis were examined by 5'-nase-alkaline phosphatase (5'-NA-ALP) double enzyme-histochemistry and
Species identification and molecular characterization of Acanthamoeba isolated from contact lens paraphernalia.
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We applied ribosomal RNA gene (rDNA) polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to identify Acanthamoeba isolates from contact lens paraphernalia, and characterized these on the basis of mitochondrial DNA (mtDNA) RFLP and isoenzyme analysis. The 22 Acanthamoeba
Nontuberculous mycobacterial infections in King Chulalongkorn Memorial Hospital.
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BACKGROUND
Nontuberculous mycobacteria (NTM) can cause infections in both human immunodeficiency virus (HIV)-infected and HIV-noninfected patients. The incidence of NTM infections has been increasing since the acquired immunodeficiency syndrome (AIDS) epidemics. However, the epidemiologic and
Expressed sequence tags (ESTs) analysis of Acanthamoeba healyi.
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Randomly selected 435 clones from Acanthamoeba healyi cDNA library were sequenced and a total of 387 expressed sequence tags (ESTs) had been generated. Based on the results of BLAST search, 130 clones (34.4%) were identified as the genes encoding surface proteins, enzymes for DNA, energy production
Detection of herpes simplex virus type 1 in herpetic ocular diseases by DNA-DNA hybridization using a biotinylated DNA probe.
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A diagnostic hybridization assay for detecting herpes simplex virus type 1 (HSV-1) in ocular specimens was developed using cloned viral DNA as a probe. This hybridization assay is based on visualizing a biotinylated probe that is hybridized to the target DNA by a streptavidin/alkaline phosphatase
Lymphatic vessels correlate closely with inflammation index in alkali burned cornea.
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OBJECTIVE
To study the relationship between corneal lymphangiogenesis and inflammation in alkali burned corneas.
METHODS
Rat corneal lymphatic and blood vessels were labeled and distinguished by whole mount immunofluorescence and 5'-nase-alkaline phosphatase (5'-NA-ALP) double enzyme-histochemistry.
Lysosomal enzymes in tear fluids from patients with ocular diseases.
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Lysosomal enzyme activities in the tear fluids were determined in patients with ocular diseases. Acid phosphatase, beta-D-glucuronidase, N-acetyl-beta-D-glucosaminidase, and beta-D-mannosidase activities were almost the same among the tear fluids from patients with myopia, rhegmatogenous retinal
Osteomalacia as a presenting manifestation of Sjögren's syndrome.
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Osteomalacia is still common in Morocco, where the leading causes are nutritional deficiencies followed by intestinal diseases. Osteomalacia rarely occurs as the first manifestation of a renal tubule disorder due to a connective tissue disease such as Sjögren's syndrome. The case of a 40-year-old
Species identification and characterization of an Acanthamoeba strain from human cornea.
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The isoenzyme pattern of an Acanthamoeba, stock H-1, isolated from a patient with keratitis (Krankenhaus Heidberg, Hamburg) was compared with that of two strains of A. quina-A. lugdunensis (302-2, 312-1), two stocks of A. lenticulata (45, 89-1) and one strain of A. rhysodes (302-1). The isolated
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