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Qualitative and quantitative detection of honey adulterated with high-fructose corn syrup and maltose syrup by using near-infrared spectroscopy.
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Near-infrared spectroscopy (NIR) was used for qualitative and quantitative detection of honey adulterated with high-fructose corn syrup (HFCS) or maltose syrup (MS). Competitive adaptive reweighted sampling (CARS) was employed to select key variables. Partial least squares linear discriminant
An immobilized two-enzyme system (fungal alpha-amylase/glucoamylase) and its use in the continuous production of high conversion maltose-containing corn syrups.
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Fungal alpha-amylase (E.C. 3.2.1.1) and glucoamylase (E.C. 3.2.1.3) were chemically attached to separate reactor modules made from Microporous Plastic Sheet (MPS). Immobilization of enzymes and subsequent chemical reactions were accomplished by pumping reactants through the sheet, i.e.,
Respiration of Sugars in Spinach (Spinacia oleracea), Maize (Zea mays), and Chlamydomonas reinhardtii F-60 Chloroplasts with Emphasis on the Hexose Kinases.
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The role of hexokinase in carbohydrate degradation in isolated, intact chloroplasts was evaluated. This was accomplished by monitoring the evolution of 14CO2 from darkened spinach (Spinacia oleracea), maize (Zea mays) mesophyll, and Chlamydomonas reinhardtii chloroplasts externally supplied with
Purification and Properties of Mesophyll and Bundle Sheath Cell alpha-Glucan Phosphorylases from Zea mays L. : Equivalence of the Enzymes with the Cytosol and Plastid Phosphorylases from Spinach.
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Two major alpha-glucan phosphorylases (I and II) from leaves of the C(4) plant corn (Zea mays L.) were previously shown to be compartmented in mesophyll and bundle sheath cells, respectively (C Mateyka, C Schnarrenberger 1984 Plant Sci Lett 36: 119-123). The two enzymes were separated by
Effects of corn syrup solids containing maltobionic acid (maltobionic acid calcium salt) on bone resorption in healthy Japanese adult women: A randomized double-blind placebo-controlled crossover study.
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Maltobionic acid is known to have an inhibitory effect on the differentiation of osteoclasts, and it has also been reported in an intervention trial that ingestion of corn syrup solids containing maltobionic acid maintained and increased the bone density of postmenopausal women. However, there is no
Molecular cloning and expression in Escherichia coli of an active fused Zea mays L. D-amino acid oxidase.
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D-Amino acid oxidase (DAAO) is an FAD-dependent enzyme that metabolizes D-amino acids in microbes and animals. However, such ability has not been identified in plants so far. We predicted a complete DAAO coding sequence consisting of 1158 bp and encoding a protein of 386 amino acids. We cloned this
Cloning and biochemical characterization of an anionic peroxidase from Zea mays.
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We have isolated, cloned and characterized a cDNA from Zea mays L., denoted ZmAP1, coding for an anionic peroxidase. The open reading frame of ZmAP1 starting 72 residues from the 5' end of the cDNA predicts a 37,778 dalton protein of 356 amino acid residues. The protein has high similarity to other
Solvent and viscosity effects on the rate-limiting product release step of glucoamylase during maltose hydrolysis.
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Release of product from the active site is the rate-limiting step in a number of enzymatic reactions, including maltose hydrolysis by glucoamylase (GA). With GA, an enzymatic conformational change has been associated with the product release step. Solvent characteristics such as viscosity can
Fructose content in popular beverages made with and without high-fructose corn syrup.
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OBJECTIVE
Excess fructose consumption is hypothesized to be associated with risk for metabolic disease. Actual fructose consumption levels are difficult to estimate because of the unlabeled quantity of fructose in beverages. The aims of this study were threefold: 1) re-examine the fructose content
First Report of Ear Soft Rot of Corn (Zea mays) Caused by Burkholderia gladioli in the United States.
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During the summer of 2005, an uncharacterized disease was observed on sweet corn 'Mirai 301BC' commercially grown in Sunflower County, Mississippi. Initial symptoms developing at the base of the ear on interior husk leaves were brown, water-soaked, irregular lesions. These gradually enlarged up to
On the use of differential solubility in aqueous ethanol solutions to narrow the DP range of food-grade starch hydrolysis products.
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Considerable research is focused on understanding the functionality of starch hydrolysis products (SHP) consisting of glucose, maltose, maltooligosaccharides (MOS), and maltopolysaccharides (MPS). A confounding factor in this research is the high molecular dispersity of commercially available SHP.
Sugar uptake by maize endosperm suspension cultures.
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Maize (Zea mays L.) endosperm suspension cultures are a useful model system for studying biochemical and physiological events in developing maize endosperm. In this report, sugar uptake by the cultures is characterized. Uptake of (14)C-labeled fructose and l-glucose was linear with time, while the
Improved Expression and Purification of the Carotenoid Biosynthetic Enzyme Z-ISO.
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Carotenoids are a large class of pigments that are essential for survival of plants and other species that consume these plant-derived compounds and their bioactive derivatives. The plant biosynthetic pathway is nuclear-encoded and localized in plastids. The pathway enzymes had been known for many
Elevated curdlan production by a mutant of Agrobacterium sp. ATCC 31749.
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A mutant strain of the curdlan-producing bacterium Agrobacterium sp. ATCC 31749, isolated by ethylmethane sulfonate mutagenesis and resistance to ampicillin, was capable of elevated curdlan synthesis. Using 2.5% corn syrup, glucose or maltose as a carbon source, the mutant strain was shown to
[Cariogenic carbohydrates in maltodextrin-containing breast milk food substitutes].
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Ten infant formulas containing maltodextrin were analysed for fermentable carbohydrates using enzymatic analysis test-combinations and a spectrophotometer. Besides lactose, sucrose and fructose an assessment was made of sugars typically contained in maltodextrin and corn syrup, namely maltose,
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