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nitrosodimethylamine/majs
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10 resultater
The effects of dietary corn oil on the metabolism and mutagenic activation on N-nitrosodimethylamine (DMN) by hepatic microsomes from male and female rats.
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Microsomes from male and female rats fed a diet containing 10% corn oil metabolized N-nitrosodimethylamine (DMN) more rapidly than microsomes from rats fed a similar diet devoid of corn oil. The daily administration of phenobarbital for 4 days prior to harvesting the microsomes resulted in
Roles of dietary corn oil in the regulation of cytochromes P450 and glutathione S-transferases in rat liver.
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To study the molecular mechanisms by which dietary lipids affect the levels of cytochrome P450 (P450) isozymes, male Sprague-Dawley rats were fed either fat-free (FF) or 20% corn oil (CO) diet in combination with one of the following three treatments: no inducer, phenobarbital (PB) and acetone.
Role of dietary corn oil in the function of hepatic drug and carcinogen metabolizing enzymes of starved-refed rats: response to the mixed function oxidase inducer, 3-methylcholanthrene.
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Hepatic microsomes from rats starved 48 hours and refed diets containing zero, 3 or 20% corn oil metabolized benzo(a)pyrene, aniline and N-nitrosodimethylamine in proportion to the quantity of corn oil in the diet. No diet-related changes in apparent Km for these reactions were evident. The content
Differential effects of garlic oil and its three major organosulfur components on the hepatic detoxification system in rats.
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The objective of this study was to compare the modulatory effect of garlic oil and its three organosulfur compounds, diallyl sulfide (DAS), diallyl disulfide (DADS), and diallyl trisulfide (DATS), on rat hepatic detoxification enzyme activity, and protein and mRNA expression. Rats were orally
The in vivo-in vitro hepatocyte assay for assessing DNA repair and DNA replication: studies in the CD-1 mouse.
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The in vivo-in vitro hepatocyte assay, a short-term test that is useful for detecting potential rodent hepatocarcinogens, has been extended to the CD-1 mouse. Male CD-1 mice, 50-100 days old, were dosed orally with N-nitrosodimethylamine (NDMA), trichloroethylene (TCE), 2-acetylaminofluorene
Regulation of hepatic microsomal cytochrome P450IIE1 level by dietary lipids and carbohydrates in rats.
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The present work tests the hypothesis that high fat/low carbohydrate diets elevate the level of liver microsomal cytochrome P450IIE1. Male Sprague-Dawley rats were fed liquid diets containing varied ratios of corn oil/carbohydrate for 4 d. Rats fed diets with higher fat/carbohydrate ratios produced
Dietary fat--a requirement for induction of mixed-function oxidase activities in starved-refed rats.
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Male rats were starved 0-48 hr, and then refed diets containing 0% (F.F.) to 20% corn oil (C.O.) lab chow or 20% coconut oil (C.C.O.) for 1-4 days. Some received phenobarbital sodium (80 mg/kg, i.p. daily) for 1-3 days prior to decapitation. Five cytochrome P-450-dependent indicators were assayed as
Effects of dietary restriction and fasting on selected rat liver enzymes of xenobiotic metabolism and on AOM-induced DNA guanine methylation in rat liver and colon.
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Using five- to eight-week-old male F344 rats and a high-fat (23.5% corn oil) modified AIN-76A diet, we examined the effects of dietary restriction (a 3-wk 30% reduction of food intake with respect to ad libitum-fed controls) or complete fasting (2 days without food) on the activities of hepatic
Further factors influencing N-nitrosamine formation in bacon.
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The possible relationship of unsaturated fatty acids in adipose tissue to the formation of N-nitrosamines in bacon was evaluated by trials in which pigs were fed regular (control), tallow-, coconut fat- and corn oil-supplemented diets. Bacon prepared from pigs fed corn oil-supplemented diets
Modulation of the levels of cytochromes P450 in rat liver and lung by dietary lipid.
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This study was undertaken to investigate the effect of dietary lipid on the regulation of several constitutive P450 isozymes. Male Sprague-Dawley rats with body weights of 130-140 g were fed either a 20% corn oil (CO) diet or a fat-free (FF) diet for 4 days following 2 days of fasting. Using liver
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