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phytohemagglutinin/dental caries
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The effect of phytohemagglutinin on phagocytosis in the mouse peritoneal cavity.
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[Cytobiological research in a case of progressive myeloid aplasia. 3. Injection into the medullary cavities of stem cells obtained from autologous lymphocytes activated in culture with phytohemagglutinin].
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Properties of three monoclonal antibodies that recognize an 80-kDa phytohemagglutinin-binding glycoprotein from porcine lymphocytes.
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Monoclonal antibodies (mAbs) directed against porcine splenocyte phytohemagglutinin receptor glycoproteins were produced in BALB/c mice. Three antibody-producing, stable hybridomas were cloned and expanded in the peritoneal cavity of BALB/c mice. The mAbs (A7, B1, and H3) were purified and belong to
Cell-mediated immune status in patients with squamous cell carcinoma of the oral cavity.
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Sixteen untreated patients with squamous cell carcinoma of the oral cavity were tested for in vitro immune status in comparison with the normal healthy donors. The parameters investigated were total leukocyte and lymphocyte counts, percentages and absolute counts of T- and B-cells in circulation,
Phytohemagglutinin gene expression during seed development of the runner bean, Phaseolus coccineus.
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The expression of phytohemagglutinin (PHA) genes was studied in various tissues during the development of the seeds of Phaseolus coccineus cv. Hammond's Dwarf Scarlet by means of northern hybridization and reverse transcription polymerase chain reaction (RT-PCR). The expression is highly
Lymphocyte reactivity in patients with cancer of the oral cavity measured by sister chromatid differential staining.
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Lymphocyte reactivity in phytohemagglutinin-stimulated peripheral lymphocyte cultures from 22 patients with squamous cell carcinoma of the oral cavity and 29 age- and sex-matched controls was measured by the bromodeoxyuridine-Giemsa method for demonstrating sister chromatid differential staining.
Effect of hemostatics used during operations for digestive organ on cancer cells present in the peritoneal cavity.
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We investigated effects of hemostatics used during operations for digestive organ on cancer cells present in the peritoneal cavity using BALB/c mice inoculated with Meth A tumor cells (fibrosarcoma) intraperitoneally (i.p.) and C3H/He mice inoculated with MH134 tumor cell (hepatic cell carcinoma)
Studies on phytohemagglutinin-induced peritonitis of mice: dynamics of inflammatory responses and influence of anticomplementary agents and anti-inflammatory drugs.
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The intraperitoneal injection of phytohemagglutinin in the mouse resulted in the marked accumulation of leukocytes, especially macrophages, in the peritoneal cavity between 8 and 36 h after the injection. Parallel to the accumulation of macrophages, exudation of plasma proteins into the peritoneal
Phytohemagglutinin improves the development and ultrastructure of in vitro-cultured goat (Capra hircus) preantral follicles.
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The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles cultured in vitro. Secondary follicles (∼0.2 mm) were isolated from the cortex of caprine ovaries and cultured individually for 6 days in α-MEM+
[Phytohemagglutinin-stimulated lymphocyte proliferation in a diffusion chamber in vivo].
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Lymphocyte proliferation stimulated with phytohaemagglutinin (PHA) was studied in vivo. For control aims experiments were done also lymphocytes without PHA. In the investigations the technique of diffusion chamber was used. Chambers with lymphocytes (1 X 10(6) cells in a chamber) were implanted into
Interleukin-2 mediated regulation of mitogen-activated T cell reactivity from different lymphoid sources in patients with squamous cell carcinoma of the oral cavity.
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Peripheral blood lymphocytes (PBL) from patients with oral cancer (treated and untreated), oral leukoplakia and healthy donors; lymphocytes from metastatic and non-metastatic lymph nodes (met LNL and non-met LNL); and tumor infiltrating lymphocytes (TIL) were tested for proliferative response to
[Observations on the local administration of autologous lymphokine activated killer cells and recombinant interleukin-2 in patients with malignant gliomas].
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Recently lymphocytes from patients with cancer have proved to be activated by interleukin 2 (IL-2), and show a strong cytotoxicity. On the basis of this fact, we have tried to inject lymphokine activated killer (LAK) cells and recombinant IL-2 (rIL-2) directly into the cavity of brain tumor. We
Periodontal bone loss and immune characteristics of congenitally athymic and thymus cell-reconstituted athymic rats.
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We investigated the ability to reconstitute the T-cell deficiency in congenitally athymic (nude; rnu/rnu) Rowett rats by intravenous injection of 10(7) heterozygous normal (rnu/+) rat thymus cells. The thymus cell-reconstituted animals showed essentially normal lymphocyte proliferation to
Selective depletion of rat neutrophils by in vivo administration of a monoclonal antibody.
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A monoclonal antibody (RP-3) that depletes rat neutrophils selectively in vivo was developed by hybridization of mouse myeloma cells (P3-X63.Ag8.653) and spleen cells of BALB/c mice sensitized with peritoneal neutrophils of WKA/Hok rats. RP-3 reacted with rat neutrophils but not with lymphocytes,
[Infectious complications after esophageal surgery].
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The incidence of wound infection, which is an intrasurgical field infection, is lower than the incidence of pneumonia, which is an extrasurgical field infection, after esophageal cancer surgery. Several trials predicting postoperative infectious complications have been reported. One measured the
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