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phytolacca/carbohydrate
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Glycocalyx carbohydrates of uterine epithelial cells increase during early pregnancy in the rat.
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Carbohydrates were studied on the surface of uterine epithelial cells by lectin-avidin-ferritin histochemistry. The lectins, wheat germ agglutinin, fucose binding protein, soy bean agglutinin and the lectin from Phytolacca americana were used in the study. Statistically significant increases in
Alterations in cell-surface carbohydrates of rat large granular lymphocytes associated with interleukin-2 activation.
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The activation of large granular lymphocytes (LGLs)/natural killer (NK) cells with interleukin-2 (IL-2) has been shown to increase the ability of these cells to lyse NK-resistant tumor target cells. Activated LGLs, termed LAK (lymphokine-activated killer) cells, have been demonstrated to be of
Similarity between protein-protein and protein-carbohydrate interactions, revealed by two crystal structures of lectins from the roots of pokeweed.
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The roots of pokeweed (Phytolacca americana) are known to contain the lectins designated PL-A, PL-B, PL-C, PL-D1, and PL-D2. Of these lectins, the crystal structures of two PLs, the ligand-free PL-C and the complex of PL-D2 with tri-N-acetylchitotriose, have been determined at 1.8A resolution. The
Carbohydrate-binding specificity of pokeweed mitogens.
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The carbohydrate-binding specificity of two pokeweed (Phytolacca americana) mitogens (Pa-1 and Pa-2) was investigated by means of hemagglutination inhibition assays and the quantitative inhibition of the binding of 125I-labeled lectins to human erythrocytes using various oligosaccharides,
Inhibition of human GLUT1 and GLUT5 by plant carbohydrate products; insights into transport specificity.
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Glucose transporters GLUT1 (transports glucose) and GLUT5 (transports fructose), in addition to their functions in normal metabolism, have been implicated in several diseases including cancer and diabetes. While GLUT1 has several inhibitors, none have been described for GLUT5. By transport activity
Lectin Profile Variation in Mesenchymal Stem Cells Derived From Different Sources
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Human mesenchymal stem cells (MSCs), a promising source of stem cells for regenerative medicine, have different morphological and functional characteristics. Carbohydrate moieties on the cell surface play an important role, including cell-cell interaction and cell recognition. The objective of this
[Studies on the physiological response of Phytolacca americana to manganese toxicity by FTIR spectroscopy].
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A technique based on Fourier transform infrared (FTIR) spectrometry was used to detect the physiological changes in chemical composition of Phytolacca americana under a wide range of Mn2+ treatments (0, 0.125, 0.25, 0.5 and 1 g x kg(-1)). Using the roots, stems and leaves of phytolacca americana,
Purification and properties of a protease from the sarcocarp of bead tree fruit.
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A protease was purified from bead tree fruit (Melia azedarach L. var. japonica Makino) in four steps, including HPLC gel-filtration. The M(r) of the enzyme, named melain, was estimated to be 25,000 on SDS-PAGE and on HPLC gel filtration. Melain contained a carbohydrate moiety. Using casein as a
Betacyanins from plants and cell cultures of Phytolacca americana.
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Betacyanins from cell cultures of Phytolacca americana were characterized and compared with those of the stems and ripening fruits of the plant. Whereas in fruits prebetanin (betanin 6'-O-sulphate) and its isoform predominate, in the stem and cell cultures feruloylated derivatives occur as the major
Structures of two lectins from the roots of pokeweed (Phytolacca americana).
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Pokeweed lectin (PL), a lectin specific for N-acetylglucosamine-containing saccharides, stimulates peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins have been isolated from the roots of pokeweed (Phytolacca americana) and shown to contain homologous
Ultrastructural studies of glycan changes in the apical surface of the uterine epithelium during pre-ovulatory and and pre-implantation stages in the marmoset monkey.
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It has been postulated that carbohydrates are involved in a variety of cell-cell interactions including blastocyst implantation. In primates, there are only limited investigations on the ultrastructural localisation of the cyclic changes in uterine epithelial surface carbohydrates. Our aim was to
Lectin histochemical characteristics of the canine female mammary gland.
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Twelve biotinylated lectins and an avidin-biotin-peroxidase method were used to detect and localize specific carbohydrate residues on formalin-fixed, paraffin-embedded female canine mammary gland sections. Histologic sections from 3 lactating and 7 nonlactating mixed-breed dogs (age 5.6 +/- 0.35
The role of the glycan moiety on the structure-function relationships of PD-L1, type 1 ribosome-inactivating protein from P. dioica leaves.
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N-glycosylation is one of the major naturally occurring covalent co-translational modifications of proteins in plants, being involved in proteins structure, folding, stability and biological activity. In the present work the influence of carbohydrate moieties on the structure-function relationships
De novo Assembly of the Pokeweed Genome Provides Insight Into Pokeweed Antiviral Protein (PAP) Gene Expression.
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Ribosome-inactivating proteins (RIPs) are RNA glycosidases thought to function in defense against pathogens. These enzymes remove purine bases from RNAs, including rRNA; the latter activity decreases protein synthesis in vitro, which is hypothesized to limit pathogen proliferation by causing
The influence of some vegetable extracts on the in vitro adherence of mouse and human lymphocytes to nylon fibers.
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We found that the total watery extracts obtained from roots of various plants such as Symphytum officinale, Phytolacca americana etc, precipitate human glycoproteins, agglutinate sheep red blood cells (SRBC) and stimulate lymphocyte adherence to nylon fibers. Five out of seven extracts precipitated
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