polygalacturonic acid/majs

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Electrical potentials in stomatal complexes.

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Guard cells of several species, but predominantly Commelina communis, were impaled by micropipette electrodes and potential differences measured that occurred between cell compartments and the flowing bathing medium. The wall developed a Donnan potential that was between -60 and -70 millivolt in 30

Enzymic dissociation of zea shoot cell wall polysaccharides : I. Preliminary characterization of the water-insoluble fraction of zea shoot cell walls.

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The water-insoluble fraction, obtained after successive treatment of an insoluble fraction of a buffer-homogenate of Zea mays L. hybrid B73 x Mo 17 shoots with 3 molar LiCl and hot water, was treated with alpha-amylase to remove starch. This fraction has been subjected to sugar composition analysis

Identification of polysaccharide hydrolases involved in autolytic degradation of zea cell walls.

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Cell walls of Zea mays (cv L.G.11) seedlings labeled with (14)C were treated with alpha-amylase from Bacillus subtilis to remove starch and mixed linkage glucans. These walls released arabinose, xylose, galactose, and galacturonic acid in addition to glucose when they were allowed to autolyze.

An exo-poly-alpha-D-galacturonosidase, PehB, is required for wild-type virulence of Ralstonia solanacearum.

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Ralstonia solanacearum, which causes bacterial wilt disease of many plant species, produces several extracellular plant cell wall-degrading enzymes that are suspected virulence factors. These include a previously described endopolygalacturonase (PG), PehA, and two exo-PGs. A gene encoding one of the

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