The rapid release of H2O2 by elicited plant cells, recently termed the oxidative burst, was investigated in suspension-cultured soybean (Glycine max Merr. cv Kent) cells stimulated with a purified polygalacturonic acid (PGA) elicitor. Examination of the elicited cells by fluorescence microscopy
Plants often respond to microbial infection by producing antimicrobial compounds called phytoalexins. Plants also produce phytoalexins in response to in vitro treatment with molecules called elicitors. Specific elicitors, including a hexa-β-glucosyl glucitol derived from fungal cell walls, the
We have employed both (31)P nuclear magnetic resonance spectroscopy and two intracellular fluorescent pH indicator dyes to monitor the pH of the vacuole and cytoplasm of suspension-cultured soybean cells (Glycine max Merr cv Kent). For the (31)P nuclear magnetic resonance studies, a flow cell was
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