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robinia/carbohydrate
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Water use strategies and drought intensity define the relative contributions of hydraulic failure and carbohydrate depletion during seedling mortality
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COMBINING HYDRAULIC: and carbon-related measurements can help elucidate drought-induced plant mortality. To study drought mortality mechanisms, seedlings of two woody species, including the anisohydric Robinia pseudoacacia and isohydric Quercus acutissima, were cultivated in a greenhouse and
Relative contributions of hydraulic dysfunction and carbohydrate depletion during tree mortality caused by drought.
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Drought-induced tree mortality has been observed worldwide. Nevertheless, the physiological mechanisms underlying this phenomenon are still being debated. Potted Robinia pseudoacacia and Platycladus orientalis saplings were subjected to drought and their hydraulic failure and carbon starvation
Studies by site-directed mutagenesis of the carbohydrate-binding properties of a bark lectin from Robinia pseudoacacia.
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A bark lectin, RBL, from Robinia pseudoacacia (black locust), binds galactose-related sugars specifically. Recombinant RBL (rRBL) with a histidine tag was expressed in Escherichia coli, purified and characterized. rRBL agglutinated rabbit erythrocytes and the hemagglutination was inhibited by
Structural basis for carbohydrate binding properties of a plant chitinase-like agglutinin with conserved catalytic machinery.
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A new chitinase-like agglutinin, RobpsCRA, related to family GH18 chitinases, has previously been identified in black locust (Robinia pseudoacacia) bark. The crystal structure of RobpsCRA at 1.85Å resolution reveals unusual molecular determinants responsible for the lack of its ancestral chitinase
Structural analysis of the carbohydrate chain of glycopeptides isolated from Robinia pseudoacacia seed lectins.
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Robinia pseudoacacia seeds contain lectins which are closely related. Pronase digestion of the dimeric and tetrameric lectins, RPA1 and RPA3, gave glycopeptides. The structure of the oligosaccharide was determined by 1H NMR spectroscopy and carbohydrate determination as
Carbohydrate-binding proteins from the sieve-tube sap of Robinia pseudoacacia L.
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Evidence is provided for the occurrence of lectins (phytohemagglutinins) in the sieve-tube sap of Robinia pseudoacacia L. The most potent sugars found to inhibit the complex formation of the soluble protein with rabbit erythrocytes were N-acetyl-galactosamine and glycosides containing galactose.
[Carbohydrate contents of phytohemagglutinin from Robinia pseudo acacia and their role in the erythro agglutinating activity].
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Studies on the Chemistry of the Living Bark of the Black Locust in Relation to Its Frost Hardiness. V. Seasonal Transformations and Variations in the Carbohydrates: Starch-Sucrose Interconversions.
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Improvement and transcriptome analysis of root architecture by overexpression of Fraxinus pennsylvanica DREB2A transcription factor in Robinia pseudoacacia L. 'Idaho'.
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Transcription factors play a key role to enable plants to cope with abiotic stresses. DREB2 regulates the expression of several stress-inducible genes and constitutes major hubs in the water stress signalling webs. We cloned and characterized a novel gene encoding the FpDREB2A transcription factor
Phylogenetic diversity of rhizobial strains nodulating Robinia pseudoacacia L.
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Lack of knowledge exists regarding the diversity of rhizobial strains nodulating black locust (Robinia pseudoacacia L.), which is a neophytic tree species widely distributed in Europe. Seventeen rhizobial strains isolated from nodules of black locust at a German location were examined by phenotypic
Studies on the interaction of dopamine beta-hydroxylase from various sources with phytohaemagglutinins.
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1. Bovine adrenal medulla dopamine beta-hydroxylase, a glycoprotein with terminal mannose residues in carbohydrate moiety, did not precipitate with any lectins tested except concanavalin A. After digestion with neuraminidase, the enzyme was shown to interact with ricin, and a good correlation was
Diversity among Rhizobia Effective with Robinia pseudoacacia L.
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The diversity of rhizobia that form symbioses with roots of black locust (Robinia pseudoacacia L.), an economically important leguminous tree species, was examined by inoculating seedling root zones with samples of soil collected from the United States, Canada, and China. Bacteria were isolated from
Purification and characterization of Robinia pseudoacacia seed lectins. A re-investigation.
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Two lectins, RPA 1 and RPA 3, were purified from Robinia pseudoacacia seeds. These two lectins differ in their physicochemical and biological properties. By analytical ultracentrifugation the Mr values of RPA 1 and RPA 3 were estimated to be 59,000 and 105,000 respectively. From
Flavonoid insertion into cell walls improves wood properties.
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Wood has an excellent mechanical performance, but wider utilization of this renewable resource as an engineering material is limited by unfavorable properties such as low dimensional stability upon moisture changes and a low durability. However, some wood species are known to produce a wood of
Affinity chromatography of a lectin from Robinia pseudoacacia L. and demonstration of lectins in sieve-tube sap from other tree species.
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A carbohydrate-binding protein in the sievetube sap of Robinia pseudoacacia L. was isolated by affinity chromatography on D-galactosamine coupled to CH-Sepharose 4B. The yield of the purified protein was 41,3 μg/ml of sieve-tube sap or 5,7% of the total protein. In disk electrophoresis at an
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