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14 resultater
Antigenicity of a filarial protease from Setaria digitata in Wuchereria bancrofti infection.
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A protease isolated from adult Setaria digitata revealed a single, 110-kDa band in a gelatin-impregnated, substrate gel. Analysis with specific inhibitors indicated it to be a metallo-protease. IgG from chronic filarial patients living in an area of Orissa, India, endemic for Wuchereria bancrofti
Screening of different classes of proteases in microfilarial and adult stages of Setaria cervi.
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Many of the filarial proteases involved in critical physiological functions are expressed in stage-specific manner and belong to various mechanistic classes. Setaria cervi, a bovine filarial parasite express different classes of proteases. This parasite shows strong antigenic cross-reactivity with
Partial characterization of affinity purified excretory-secretory protease from Setaria digitata.
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Excretory-secretory protease of S. digitata released along with the microfilariae (mf) during hatching has been purified by affinity chromatography. No other activity could be detected in the affinity purified material. Homogeneity is checked by native PAGE. It has a pH optimum of 5.4 and a
Vaccination with Setaria cervi 175 kDa collagenase induces high level of protection against Brugia malayi infection in jirds.
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A zinc containing metalloprotease, 175 kDa collagenase, purified from adult female Setaria cervi showed strong cross-reactivity with sera from putatively immune (PI) individuals (unpublished observation) and induced cytotoxicity to B. malayi L3 larvae and microfilariae by ADCC mechanism [Srivastava
Tissue localization of collagenase and leucine aminopeptidase in the bovine filarial parasite Setaria cervi.
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BACKGROUND
Like other helminth proteases, filarial proteases have also been shown to require for parasite survival inside the host and mediate various physiologic processes such as tissue invasion, feeding, embryogenesis and host immune evasion. Many of these proteases have shown potential for
Identification and characterization of a novel prolyl oligopeptidase in filarial parasite Setaria cervi.
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A 75 kDa serine protease having prolyl oligopeptidase activity has been purified from Setaria cervi, a bovine filarial parasite. The MALDI-MS/MS analysis of the purified protein revealed 6 peptides showing nearest match S9A (prolyl oligopeptidase) family protein from Plesiocystis pacifica. The ScPOP
Thioredoxin reductase from the bovine filarial parasite Setaria cervi: Studies on its localization and optimization of the extraction.
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Exploration of novel drug targets has been the major thrust area in filarial research. In this regard, identification and characterization of oxidative enzymes that play pivotal role in the survival of filarial parasite inside host are of immense importance. In this study, we are reporting the
Setaria cervi: in vitro released collagenases and their inhibition by Wuchereria bancrofti infected sera.
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In vitro released products of adult Setaria cervi females, microfilariae and extracts showed considerable amounts of collagenase activity. On the basis of per mg protein released in vitro, the products of both microfilariae and adult females exhibited comparable activity but this was much higher
Combination of DEC plus aspirin induced mitochondrial mediated apoptosis in filarial parasite Setaria cervi.
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Diethylcarbamazine (DEC) is the main drug used against lymphatic filariasis but it is only microfilaricidal. Hence there is an urgent need for adulticidal drug. Aspirin is known nonsteroidal anti-inflammatory drugs which can inhibit prostaglandin H synthase and also induces apoptosis. Studies
Effect of Extremely Low Power Time-Varying Electromagnetic Field on Germination and Other Characteristics in Foxtail Millet (Setaria italica) Seeds
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The ability of extremely low, time-varying electromagnetic field (EMF) to improve germination efficacy was studied in Foxtail millet (Setaria italica) seeds using response surface methodology. An optimal factorial central composite design was chosen to optimize the EMF with three critical factors,
In vitro release of biologically active materials from the bovine filarial parasite Setaria digitata.
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Release of macromolecules by S. digitata, in 9 different media under in vitro condition have been studied. A direct relationship between microfilariae (mf) release and associated folin positive materials was seen in majority of the cases. High activities of hydrolytic enzymes such as protease,
Effect of acetylcholine, L-glutamine and diethyl carbamazine citrate on the release of microfilariae from Setaria digitata.
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Release of microfilariae from adult filarial parasite S. digitata was reversibly arrested in presence of 2 mM L-glutamine. Diethylcarbamazine also showed a similar effect, but at a lower concentration (0.26 mM). Both compounds inhibited activities of protease and leucine amino peptidase enzymes. The
The complete amino acid sequence of a major trypsin inhibitor from seeds of foxtail millet (Setaria italica).
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The complete amino acid sequence of a major trypsin inhibitor (FMTI-II) from seeds of foxtail millet (Setaria italica) was determined by analysis of peptides derived from the reduced and S-carboxymethylated protein by digestion with TPCK-trypsin and Staphylococcus aureus V8 protease. FMTI-II
Spatial and temporal activity of the foxtail millet (Setaria italica) seed-specific promoter pF128.
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CONCLUSIONS
pF128 drives GUS specifically expressed in transgenic seeds of foxtail millet and Zea mays with higher activity than the constitutive CaMV35S promoter and the maize seed-specific 19Z promoter. Foxtail millet (Setaria italica), a member of the Poaceae family, is an important food and
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