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8 resultater
Thymidine kinases share a conserved function for nucleotide salvage and play an essential role in Arabidopsis thaliana growth and development.
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Thymidine kinases (TKs) are important components in the nucleotide salvage pathway. However, knowledge about plant TKs is quite limited. In this study, the molecular function of TKs in Arabidopsis thaliana was investigated. Two TKs were identified and named AtTK1 and AtTK2. Expression of both genes
Cell-cycle dependency of radiosensitivity and mutagenesis in fertilized egg cells of rice, Oryza sativa L. : 1. Autoradiographic determination of the first DNA synthetic phase.
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To determine the time and duration of the first and second DNA synthetic phases in fertilized egg cells and central cells of rice, a total of 753 ovules were sampled at 2 h intervals during the first 30 h after pollination and exposed to (3)H-thymidine for 2 h at 25 °C. Autoradiographic observation
Isolation, molecular and biological properties of a lectin from rice embryo: relationship with wheat germ agglutinin properties.
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Rice lectin (Oryza sativa, var. Balilla 28) was purified from defatted embryos by aqueous acid extraction at pH 1.3 followed by ammonium sulfate precipitation between 2 and 4 M, affinity chromatography on agarose-p-aminophenyl-beta-D-N-acetylglucosamine, and gel filtration on AcA 54. The homogeneity
Gibberellin-induced growth and regulation of the cell division cycle in deepwater rice.
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Excised stem sections of deepwater rice (Oryza sativa L., cv. Pin Gaew 56) containing the highest internode were used to study induction of rapid internodal elongation by gibberellin. It has been shown previously that this growth response is based on an increased cell production rate in the
Structure and Function of the Golgi Complex in Rice Cells (II. Purification and Characterization of Golgi Membrane-Bound Nucleoside Diphosphatase).
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Inosine diphosphatase bound to Golgi membranes was studied in rice (Oryza sativa L. cv Nipponkai) cells. The enzyme was solubilized with Triton X-100 from isolated rice Golgi membranes and was highly purified employing a series of chromatography steps in the presence of 20% glycerol and 0.1% Triton
The cellular basis of the elongation response in submerged deep-water rice.
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The cellular basis of internode elongation was studied in intact deep-water rice plants (Oryza sativa L. cv. "Habiganj Aman II") and in isolated stem sections. In intact plants, growth was stimulated by submergence in water and by ethylene treatment. In isolated sections, growth was enhanced by
Heat-inducible rice hsp82 and hsp70 are not always co-regulated.
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We have characterized several heat-shock-induced genes in rice (Oryza sativa L.) and compared their expression under a variety of conditions. Three of these genes, which are analogs of the hsp82/90 family, lie within a cloned 18-kilobase (kb) region of the genome. The middle member of this cluster,
A flow cytometric method for estimating S-phase duration in plants.
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The duration of the DNA synthesis stage (S phase) of the cell cycle is fundamental in our understanding of cell cycle kinetics, cell proliferation, and DNA replication timing programs. Most S-phase duration estimates that exist for plants are based on indirect measurements. We present a method for
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