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6 resultater
A vicilin-like seed storage protein, PAP85, is involved in tobacco mosaic virus replication.
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One striking feature of viruses with RNA genomes is the modification of the host membrane structure during early infection. This process requires both virus- and host-encoded proteins; however, the host factors involved and their role in this process remain largely unknown. On infection with Tobacco
Heterologous Reconstitution of Omega-3 Polyunsaturated Fatty Acids in Arabidopsis.
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Reconstitution of nonnative, very-long-chain polyunsaturated fatty acid (VLC-PUFA) biosynthetic pathways in Arabidopsis thaliana was undertaken. The introduction of three primary biosynthetic activities to cells requires the stable coexpression of multiple proteins within the same cell. Herein, we
Sustained root culture for generation and vegetative propagation of transgenic Arabidopsis thaliana.
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Excised roots of wild-type and nitrate-reductase deficient mutant Arabidopsis thaliana (L.) HEYNH. can be propagated as sustained root cultures in liquid medium. Culture initiation from a single seedling required a two-day indoleacetic acid treatment at 0.05 mg/l concentration. Indoleacetic acid
A stress-associated protein containing A20/AN1 zing-finger domains expressed in Medicago truncatula seeds.
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MtSAP1 (Medicago truncatula stress-associated protein 1) was revealed as a down-regulated gene by suppressive subtractive hybridization between two mRNA populations of embryo axes harvested before and after radicle emergence. MtSAP1 is the first gene encoding a SAP with A20 and AN1 zinc-finger
Differential manifestation of seed mortality induced by seed-specific expression of the gene for diphtheria toxin A chain in Arabidopsis and tobacco.
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A pea vicilin promoter-diphtheria toxin A (DTx-A) chain gene fusion was introduced into Arabidopsis and tobacco. The chimeric Dtx-A gene behaves as a dominant, seed-lethal, Mendelian factor, and the segregation ratios are consistent with the numbers of integrated copies as revealed by Southern
The influences of two plant nuclear matrix attachment regions (MARs) on gene expression in transgenic plants.
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Nuclear matrix attachment regions (MARs) are thought to influence gene expression by anchoring active chromatin to the proteinaceous nuclear matrix. In this study, two plant DNA fragments with strong MAR activity were selected and tested for their effects on expression of a linked reporter gene in
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