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willow/carbohydrate
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Cellulose production based on hemicellulose hydrolysate from steam-pretreated willow.
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The production cost of cellulolytic enzymes is a major contributor to the high cost of ethanol production from lignocellulosics using enzymatic hydrolysis. The aim of the present study was to investigate the cellulolytic enzyme production of Trichoderma reesei Rut C 30, which is known as a good
Protein storage and root:shoot reallocation provide tolerance to damage in a hybrid willow system.
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To determine the mechanistic basis of tolerance, we evaluated six candidate traits for tolerance to damage using F(2) interspecific hybrids in a willow hybrid system. A distinction was made between reproductive tolerance and biomass tolerance; reproductive tolerance was designated as a plant's
Poplar males and willow females exhibit superior adaptation to nocturnal warming than the opposite sex.
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The Salicaceae family consists of dioecious woody plants. Morphological and physiological species-related, sex-specific responses to nocturnal warming in these plants are seldom-reported. In order to explore the different responses of sex-biased species to nighttime warming, males and females of
Environmental life cycle assessment of producing willow, alfalfa and straw from spring barley as feedstocks for bioenergy or biorefinery systems.
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The current study aimed at evaluating potential environmental impacts for the production of willow, alfalfa and straw from spring barley as feedstocks for bioenergy or biorefinery systems. A method of Life Cycle Assessment was used to evaluate based on the following impact categories: Global Warming
Monitoring carbohydrate enzymatic reactions by quantitative in vitro microdialysis.
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On-line in vitro microdialysis (MD) sampling followed by HPLC separation and UV absorbance detection (HPLC-UV) was used to monitor carbohydrate enzyme systems. Fundamental parameters (i.e., K(m) and V(max)) of hydrolysis reactions of 4-nitrophenyl-beta-D-glucopyranoside,
RNA-sequencing reveals the complexities of the transcriptional response to lignocellulosic biofuel substrates in Aspergillus niger.
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BACKGROUND
Saprobic fungi are the predominant industrial sources of Carbohydrate Active enZymes (CAZymes) used for the saccharification of lignocellulose during the production of second generation biofuels. The production of more effective enzyme cocktails is a key objective for efficient biofuel
RNA-sequencing reveals the complexities of the transcriptional response to lignocellulosic biofuel substrates in Aspergillus niger.
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BACKGROUND
Saprobic fungi are the predominant industrial sources of Carbohydrate Active enZymes (CAZymes) used for the saccharification of lignocellulose during the production of second generation biofuels. The production of more effective enzyme cocktails is a key objective for efficient biofuel
Use of hemicellulose hydrolysate for beta-glucosidase fermentation.
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Hydrolysis of cellulose by Trichoderma cellulases often results in a mixture of glucose, cellobiose, and low-mol-wt cellodextrins. Cellobiose is nonfermentable for most yeasts, and therefore it has to be hydrolyzed to glucose by beta-glucosidase prior to ethanol fermentation. In the present study,
Use of hemicellulose hydrolysate for beta-glucosidase fermentation.
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Hydrolysis of cellulose by Trichoderma cellulases often results in a mixture of glucose, cellobiose, and low-mol-wt cellodextrins. Cellobiose is nonfermentable for most yeasts, and therefore it has to be hydrolyzed to glucose by beta-glucosidase prior to ethanol fermentation. In the present study,
Hydrothermal treatment followed by enzymatic hydrolysis and hydrothermal carbonization as means to valorise agro- and forest-based biomass residues.
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The suitability of several abundant but underutilized agro and forest based biomass residues for hydrothermal treatment followed by enzymatic hydrolysis as well as for hydrothermal carbonization was studied. The selected approaches represent simple biotechnical and thermochemical treatment routes
Analysis of mono-, di- and oligosaccharides by CE using a two-stage derivatization method and LIF detection.
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A sensitive CE with LIF method has been developed for quantitative analysis of small carbohydrates. In this work, 17 carbohydrates including mono-, di- and oligosaccharides were simultaneously derivatized with 4-fluoro-7-nitrobenzofurazane (NBD-F) via a two-step reaction involving reductive
Repression of Pseudomonas putida phenanthrene-degrading activity by plant root extracts and exudates.
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The phenanthrene-degrading activity (PDA) of Pseudomonas putida ATCC 17484 was repressed after incubation with plant root extracts of oat (Avena sativa), osage orange (Maclura pomifera), hybrid willow (Salix alba x matsudana), kou (Cordia subcordata) and milo (Thespesia populnea) and plant root
Changes in Salix viminalis L. cv. 'Cannabina' morphology and physiology in response to nickel ions--hydroponic investigations.
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The aim of the study was to assess the ability of 'basket willow' to bioaccumulate nickel with simultaneous analysis of biomass parameters and biomarkers of plants physiological reaction to the metal. Cuttings of Salix viminalis L. cv. 'Cannabina' were cultivated in Knop's medium containing nickel
Effect of acetic acid and furfural on cellulase production of Trichoderma reesei RUT C30.
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Because of the high temperature applied in the steam pretreatment of lignocellulosic materials, different types of inhibiting degradation products of saccharides and lignin, such as acetic acid and furfural, are formed. The main objective of the present study was to examine the effect of acetic acid
Characterization of three plant biomass-degrading microbial consortia by metagenomics- and metasecretomics-based approaches.
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The selection of microbes by enrichment on plant biomass has been proposed as an efficient way to develop new strategies for lignocellulose saccharification. Here, we report an in-depth analysis of soil-derived microbial consortia that were trained to degrade once-used wheat straw (WS1-M),
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