[Effect of injectio Salvia Miltiorrhiza on gentamicin ototoxicity-induced activity of nitric oxide synthase in cochlear stria vascularis of guinea pig].

OBJECTIVE

to investigate the change of nitric oxide synthase (NOS) activity in cochlear stria vascularis (SV) of guinea pig after gentamicin (GM) and Salvia Miltiorrhiza (SM) injection, and to explore the protective role of injectio SM on GM ototoxicity.

METHODS

NADPH-diaphorase histochemistry staining and image quantitative analysis technique, combined with auditory brainstem response (ABR) measurement.

RESULTS

SM + GM significantly reduced NOS activity in cochlear SV and ABR threshold as compared with CM along (P < 0.01); and ABR threshold shift was in high correlation with NOS activity (rControl = -0.9464; rGM = -0.9117; rSM + GM = -0.8958; P < 0.01).

CONCLUSIONS

SM can reduce NOS activity in cochlear SV so as to alleviate GM ototoxicity, thus ameliorate hearing function.