[Effect of injection salvia miltiorrhiza on the expression of inducible nitric oxide synthase in the cochlea of guinea pig damaged by streptomycin].

OBJECTIVE

To investigate the expression of Inducible Nitric Oxide Synthase (iNOS) in the cochlea of guinea pig after streptomycin (SM) and the antagonism of Salvia Miltiorrhiza injection on SM ototoxicity.

METHODS

Light microscope, transmission electron microscope (TEM), immunohistochemical staining and image quantitative analysis technique, combined with auditory brainstem response (ABR) measurement were used.

RESULTS

After 10 days by drugs, the threshold of ABR of SM increased significantly, and the threshold of ABR of DS+ SM reduced more than that of SM (P < 0.01), the Corti's organ (CO), inner hair cell (IHC) and outer hair cell (OHC), spiral ganglion(SG), stria vascularis (SV) of SM damaged more greatly than that of DS + SM under light microscope and TEM. The result of immunohistochemical staining implied the expression of iNOS in CO, IHC and OHC, SG, SV of SM higher than that of DS+ SM.

CONCLUSIONS

The threshold of ABR increases and the expression of iNOS strengthens on SM ototoxicity. DS can reduce the increasing and inhibit the over-expression of iNOS, and reduce the damage of SM ototoxicity, which implies the protective role of DS on SM ototoxicity.