[Extraction and analysis of chemical components of essential oil in Thymus vulgaris of tissue culture].

OBJECTIVE

To extract the essential oils from the Seedlings, the Aseptic Seedlings and the Tissue Culture Seedlings of Thymus vulgaris and analyze their chemical components and the relative contents.

METHODS

The essential oils were extracted by steam distillation, the chemical components and the relative contents were identified and analyzed by gas chromatography-mass spectrometry (GC/MS) and peak area normalization method.

RESULTS

The main chemical components of essential oil in these three samples had no significant difference, they all contained the main components of essential oil in Thymus vulgaris: Thymol, Carvacrol, o-Cymene, gamma-Terpinene, Caryophyllene et al. and only had a slight difference in the relative content.

CONCLUSIONS

This study provides important theoretical foundation and data reference for further study on production of essential oil in thyme by tissue culture technology.