Zhongguo Zhongyao Zazhi 2002-Dec
[HPLC determination of strychnine and brucine in Semen Strychni and its processed products].
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Abstrakt
OBJECTIVE
This paper reports a HPLC method for determinition of strychnine and brucine in Semen Strychni and its processed products of Jiangxi method and innovated methed.
METHODS
SiO2 was used as the stationary phase, n-hexane-dichloromethane-methanol-ammonia(47.5:47.5:5:0.35) as the mobile phase, with detection wavelength of 254 nm.
RESULTS
The contents of strychnine and brucine in the processed products of Jiangxi are lower.
CONCLUSIONS
This method is accurate, simple and reliable.
