Binding Capacities and Antioxidant Activities of Lactobacillus plantarum and Pichia kudriavzevii Against Cadmium and Lead Toxicities

The cadmium and lead binding capacities and antioxidant activities of Lactobacillus plantarum and Pichia kudriavzevii were evaluated in vitro and in vivo. Lactic acid bacteria and yeasts obtained from fermenting cassava mash and maize slurry were screened for tolerance to cadmium and lead at 500-1050 mg ml^-1 screened for probiotic potentials and antioxidant activities such as 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant properties. The in vivo studies were carried out in male Wistar rats. The strains identified as Lactobacillus plantarum ML05 and Pichia kudriavzevii FY05 demonstrated the best probiotic potentials and antioxidant activities. Alterations in antioxidant capacities were positive in the treatment groups. The histopathology displayed positive changes in the renal tubules and glomeruli, hypertrophy, with normal capsular spaces without inflammation in the kidney, while the liver sinusoids appear normal in the rats administered with L. plantarum ML05 and P. kudriavzevii FY05 while the infected and not treated showed cell necrosis induced by toxicities. Our results provided new evidence that Lactobacillus plantarum and Pichia kudriavzevii have different biological actions on the heavy metals binding capacities and antioxidant activities in experimental animals. This study suggests that these microorganisms can be considered dietary therapeutics against cadmium and lead toxicities.

Keywords: Antioxidant capacities; Cadmium toxicity; Lactobacillus plantarum; Lead toxicity; Pichia kudriavzevii.