The present invention relates to a detoxifizyme with the activity of transforming aflatoxin and the gene encodes thereof. Firstly a novel protein is isolated and purified, named aflatoxin-detoxifizyme (ADTZ), which has the activity of transforming aflatoxin. The ADTZ gene is obtained through specific primers, and the gene is purified and sequenced. The gene encoding of ADTZ is cloned from the total RNA of Armillariella tabescens. The recombinant protein is expressed and purified through various expression systems using genetic engineering methods. The said detoxifizyme has bioactivity of transforming AFB.sub.1, reducing mutagenic effects of AFB.sub.1. It has great potential for the manufacturing of feed or food and development of anti-tumor medicament.

Guangzhou Co-Win Bioengineering Co., Ltd.

Detoxifizyme with activity of transforming aflatoxin and the gene encodes thereof

A method is described using Aspergillus flavus fungal cultures, EBV or their combination to induce leukemic cell surface markers in mononuclear cells of former or current acute lymphoblastic leukemia/lymphoma patients. Detection of the leukemic transformation by methods known in the art identified patients who have or had acute lymphoblastic leukemia/lymphoma. Unlike aflatoxins, which indiscriminately induce leukemic transformation, the compositions used were specific to acute lymphoblastic leukemia/lymphoma-predisposed patients, but not other cancers or normal controls. An ELISA technique using the described fungal products or EBV and combination can detect individuals with history of leukemia and not controls. The combination of cultures of EBV and Aspergillus flavus, with and without radiation, results in the development of a new protein peak. These findings have implications for the etiology of leukemias and lymphomas.

aflatoxin b 1/krebs

Detoxifizyme with activity of transforming aflatoxin and the gene encodes thereof

Methods of inducing leukemia and lymphomas and detecting susceptibility to leukemia/lymphoma

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