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11 Ergebnisse
Use of elastase test, gelatin gel test and electrophoretic zymogram to determine virulence of Dichelobacter nodosus isolated from ovine foot rot.
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A comparative study using the elastase test, gelatin gel test and electrophoretic zymogram was conducted for virulence determination of 96 Dichelobacter nodosus isolates from clinical cases of ovine foot rot. Despite being time consuming, the elastase test provided a classification of D nodosus
Type IV fimbrial biogenesis is required for protease secretion and natural transformation in Dichelobacter nodosus.
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The objective of this study was to develop an understanding of the molecular mechanisms by which type IV fimbrial biogenesis, natural transformation, and protease secretion are linked in the ovine foot rot pathogen, Dichelobacter nodosus. We have shown that like the D. nodosus fimbrial subunit FimA,
Simultaneous detection and discrimination of virulent and benign Dichelobacter nodosus in sheep of flocks affected by foot rot and in clinically healthy flocks by competitive real-time PCR.
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Ovine foot rot caused by Dichelobacter nodosus is affecting sheep worldwide. The current diagnostic methods are difficult and cumbersome. Here, we present a competitive real-time PCR based on allelic discrimination of the protease genes aprV2 and aprB2. This method allows direct detection and
The pathogenicity and cultural characteristics of virulent, intermediate and benign strains of Bacteroides nodosus causing ovine foot-rot.
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The relationship between the cultural and biochemical characteristics of 22 strains of Bacteroides nodosus and their virulence for sheep was examined. Virulent, intermediate and benign strains were recognised. Although there was some relationship between virulence and colony morphology on hoof
IgG1 and IgG2 immunoglobulins to Bacteroides (Fusiformis) nodosus protease in infected and immunized sheep.
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Sheep experimentally infected with foot rot developed antibodies that neutralized the proteolytic activity of Bacteroides nodosus culture filtrates in vitro. Similar antibody activity developed in sheep vaccinated against foot rot. In both cases the antibody was initially only associated with the
Integration Host Factor is Essential for Biofilm formation, Extracellular enzyme, Zeamine Production and Virulence in Dickeya zeae.
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Dickeya zeae is a globally important pathogenic bacterium that infects a number of crops, including rice, maize, potato, and banana. Bacterial foot rot of rice caused by D. zeae is one of the most important bacterial diseases of rice in China and some Southeast Asian countries. To investigate the
Fusobacterial infections: an underestimated threat.
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The involvement of fusobacteria in a wide range of human and animal infection has long been recognised. Slow-growing anaerobes, often in polymicrobial culture, they are not always identified but are present mainly in the oropharynx, from where they are bloodborne to other sites or aspirated into the
Fusobacterium necrophorum infections in animals: pathogenesis and pathogenic mechanisms.
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Fusobacterium necrophorum, a Gram-negative, non-spore-forming anaerobe, is a normal inhabitant of the alimentary tract of animals and humans. Two subspecies of F. necrophorum, subsp. necrophorum (biotype A) and subsp. funduliforme (biotype B), have been recognized, that differ morphologically,
Twitching motility is essential for virulence in Dichelobacter nodosus.
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Type IV fimbriae are essential virulence factors of Dichelobacter nodosus, the principal causative agent of ovine foot rot. The fimA fimbrial subunit gene is required for virulence, but fimA mutants exhibit several phenotypic changes and it is not certain if the effects on virulence result from the
Detection of specific Treponema species and Dichelobacter nodosus from digital dermatitis (Mortellaro's disease) lesions in Swiss cattle.
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The aim of this study was to determine the prevalence of the three Treponema species as well as D. nodosus in Digital dermatitis (DD) and slurry of Swiss cattle using PCR. A total of 86 specimens from 24 farms were enrolled in the study. Slurry samples from 21 DD-affected and one unaffected farm
Pooling of interdigital swab samples for PCR detection of virulent Dichelobacter nodosus.
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Virulent ovine foot rot is a contagious foot disease. Given the development and validation of a real-time PCR to detect Dichelobacter nodosus isolates that contain the virulence-associated protease genes aprV2 and aprB2, the diagnosis of foot rot has made considerable progress. We evaluated pooling
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