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homoserine/atrophie
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Isolation and characterization of acyl homoserine lactone-producing bacteria during an urban river biofilm formation.
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The presence and diversity of acyl homoserine lactone (AHL)-producers in an urban river biofilm were investigated during 60-day biofilm formation. AHL biosensors detected the presence of AHL-producers in 1-60-day river biofilms. Screening for AHL-producers resulted in 17 Aeromonas spp., 3
Response of nitrogen removal performance, functional genes abundances and N-acyl-homoserine lactones release to carminic acid of anammox biomass.
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Carminic acid (CA) can serve as a redox mediator and influence the electron transfer process. CA dosages of 0-5 mg/L were added to anaerobic ammonia oxidation (anammox) biomass. The results illustrated that CA not only reduced the inorganic nitrogen removal efficiency, but also decreased the
Structure and product relationship analysis of acyl homoserine lactone synthases among Ensifer adhaerens reveals distinct chromosome and plasmid origins.
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Despite the conservative DNA sequences among LuxI (Acyl Homoserine Lactones synthase gene) homologs, structure-product relationship of AHL synthase remains to be elucidated. In this study, through degenerate primers and in vitro expression methods, we collected the information of the gene sequences
Involvement of Exogenous N-Acyl-Homoserine Lactones in Spoilage Potential of Pseudomonas fluorescens Isolated From Refrigerated Turbot.
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Some bacteria can modulate their spoilage potential by responding to environmental signaling molecules via the quorum sensing (QS) system. However, the ability of Pseudomonas fluorescens, the specific spoilage organism (SSO) of turbot, to response to environmental signaling molecules remains
Combination of culture-dependent and -independent methods reveals diverse acyl homoserine lactone-producers from rhizosphere of wetland plants.
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The culture-dependent method and a degenerate primer-based culture-independent method were combined in an effort to identify N-acyl homoserine lactone (AHL) producers in rhizosphere of wetland plants, Salix babylonica (willow) and Phragmites australis (reed). Overall, eight potential AHL-producing
Quorum sensing in bacterial species that use degenerate autoinducers can be tuned by using structurally identical non-native ligands.
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Many bacteria use quorum sensing (QS) to regulate cell-density dependent phenotypes that play critical roles in the maintenance of their associations with eukaryotic hosts. In Gram-negative bacteria, QS is primarily controlled by N-acylated L-homoserine lactone (AHL) signals and their cognate
Engineering Soluble Human Paraoxonase 2 for Quorum Quenching.
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Many pathogenic bacteria utilize quorum sensing (QS) systems to regulate the expression of their virulence genes and promote the formation of biofilm, which renders pathogens with extreme resistance to conventional antibiotic treatments. As a novel approach for attenuating antibiotic resistance and
Diversity and functional analysis of luxS genes in vibrios from marine sponges Mycale laxissima and Ircinia strobilina.
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Sponges harbor highly diverse and dense microbial communities, providing an environment in which bacterial signaling may be important. Quorum sensing (QS) is a cell density-dependent signaling process that bacteria employ to coordinate and regulate their gene expression. Previous studies have found
Quorum sensing primes the oxidative stress response in the insect endosymbiont, Sodalis glossinidius.
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BACKGROUND
Sodalis glossinidius, a maternally transmitted bacterial endosymbiont of tsetse flies (Glossina spp.), uses an acylated homoserine lactone (AHL)-based quorum sensing system to modulate gene expression in accordance with bacterial cell density. The S. glossinidius quorum sensing system
The role of sulfate in aerobic granular sludge process for emerging sulfate-laden wastewater treatment.
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Sulfate-rich wastewaters pose a major threat to mainstream wastewater treatment due to the unpreventable production of sulfide and associated shift in functional bacteria. Aerobic granular sludge could mitigate these challenges in view of its high tolerance and resilience against changes in various
Computer-aided design of agents that inhibit the cep quorum-sensing system of Burkholderia cenocepacia.
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Recent research has provided evidence that interference with bacterial cell-to-cell signaling is a promising strategy for the development of novel antimicrobial agents. Here we report on the computer-aided design of novel compounds that specifically inhibit an N-acyl-homoserine lactone-dependent
Comprehensive hippocampal metabolite responses to PM2.5 in young mice.
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Fine particulate matter (PM2.5) exposure alters brain development, clinical cognition and behavior in childhood. Previous studies of this subject have mainly been epidemiological investigations or analyses of gene and protein levels; however, gas chromatography-mass spectrometry (GC-MS)-based
Bacteria of the Roseobacter clade show potential for secondary metabolite production.
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Members of the Roseobacter clade are abundant and widespread in marine habitats and have very diverse metabolisms. Production of acylated homoserine lactones (AHL) and secondary metabolites, e.g., antibiotics has been described sporadically. This prompted us to screen 22 strains of this group for
Quorum-sensing-directed protein expression in Serratia proteamaculans B5a.
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N-Acyl-L-homoserine-lactone-producing Serratia species are frequently encountered in spoiling foods of vegetable and protein origin. The role of quorum sensing in the food spoiling properties of these bacteria is currently being investigated. A set of luxR luxI homologous genes encoding a putative
Molecular characterization of an Arabidopsis thaliana cDNA coding for a monofunctional aspartate kinase.
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A cDNA clone encoding a monofunctional aspartate kinase (AK, ATP:L-aspartate 4-phosphotransferase, EC 2.7. 2.4) has been isolated from an Arabidopsis thaliana cell suspension cDNA library using a homologous PCR fragment as hybridizing probe. Amplification of the PCR fragment was done using a
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