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15 Ergebnisse
Regulation of the maize ubiquitin (Ubi-1) promoter in developing maize (Zea mays L.) seeds examined using transient gene expression in kernels grown in vitro.
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Kernel culture was assessed for evaluating novel gene expression in developing maize (Zea mays L.) seeds by comparing the transient expression of maize ubiquitin (Ubi-1) promoter-driven β-glucuronidase (GUS) delivered by particle bombardment in kernels grown in culture with those grown in planta.
An efficient and rapid regeneration via multiple shoot induction from mature seed derived embryogenic and organogenic callus of Indian maize (Zea mays L.).
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An efficient method for in vitro micro propagation and genetic transformation of plants are crucial for both basic and applied research. Maize is one of the most important cereal crops around the world. Regeneration from immature embryo is hampered due to its unavailability round the year. On the
Plantlet regeneration from glume calli of maize (Zea mays L.).
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Totipotent callus cultures were established from anther-free glumes of 'Sweet corn', 'Seed corn,' 'DHM 103' and 'DHM 101' on MS medium supplemented with 1-2 mg/l 2,4-D. The callusing response of the glumes was tested on six different media. Glumes at the uninucleate stage of pollen development
Efficient in vitro direct shoot organogenesis from seedling derived split node explants of maize (Zea mays L.).
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Maize is one of the important cereal crops around the world. An efficient and reproducible regeneration protocol via direct organogenesis has been established using split nodes as ex-plants derived from 7 to 10 day old in vitro grown seedlings. Surface sterilized maize seeds were
Physiological Strategies to Improve the Performance of Spring Maize (Zea mays L.) Planted under Early and Optimum Sowing Conditions.
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Low temperature at stand establishment and high temperature at reproductive stage are involved in reduction of grain yield of spring maize. A field study was therefore conducted to evaluate different physiological strategies for improving performance of spring maize under temperature extremes. Seed
Kinetic and chemical analyses of the cytokinin dehydrogenase-catalysed reaction: correlations with the crystal structure.
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CKX (cytokinin dehydrogenase) is a flavoprotein that cleaves cytokinins to adenine and the corresponding side-chain aldehyde using a quinone-type electron acceptor. In the present study, reactions of maize (Zea mays) CKX with five different substrates (N6-isopentenyladenine, trans-zeatin, kinetin,
Induction and development of increased ion absorption in corn root tissue.
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Washing excised or intact primary roots of corn (Zea mays L., WF9 x M14) in aerated distilled water or dilute salt solutions for 2 hours induced doubling of the rate of accumulation of various nutrient ions and solutes. This response to washing depended upon aerobic metabolism, but involved no
O-glucosylation of cis-zeatin in maize. Characterization of genes, enzymes, and endogenous cytokinins.
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trans-Zeatin is a major and ubiquitous cytokinin in higher plants. cis-Zeatin has traditionally been viewed as an adjunct with low activity and rare occurrence. Recent reports of cis-zeatin and its derivatives as the predominant cytokinin components in some plant tissues may call for a different
Influence of Methylobacterium on iron translocation in plants.
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Iron metabolism in plants is essential to maintain optimal growth and iron nutrition is dependent on uptake of iron from the environment and movement of iron in the plant tissues. We have examined the translocation of iron in plant leaves following foliar application of FeEDTA to Vicia faba and Zea
Effects of growth substances on the absorption and transport of iron plants.
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The effects of a number of growth substances on the absorption and translocation of iron were studied in bean plants. Gibberellic acid application to the trifoliate leaf enhanced absorption of Fe applied to the primary leaf. 2-Chloroethyltrimethylammonium chloride increased absorption by the primary
Release of active cytokinin by a beta-glucosidase localized to the maize root meristem.
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A beta-glucoside encoded by a cloned Zea mays complementary DNA (Zm-p60.1) cleaved the biologically inactive hormone conjugates cytokinin-O-glucosides and kinetin-N3-glucoside, releasing active cytokinin. Tobacco protoplasts that transiently expressed Zm-p60.1 could use the inactive cytokinin
Mutations in two independent genes lead to suppression of the shoot apical meristem in maize.
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The shoot apical meristem (SAM), initially formed during embryogenesis, gives rise to the aboveground portion of the maize (Zea mays) plant. The shootless phenotype (sml) described here is caused by disruption of SAM formation due to the synergistic interaction of mutations at two genetic loci.
Plant growth hormones suppress the development of Harpophora maydis, the cause of late wilt in maize.
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Late wilt, a severe vascular disease of maize caused by the fungus Harpophora maydis, is characterized by rapid wilting of maize plants before tasseling and until shortly before maturity. The pathogen is currently controlled by resistant maize cultivars, but the disease is constantly spreading to
Enzymic synthesis of indole-3-acetyl-1-O-beta-d-glucose. II. Metabolic characteristics of the enzyme.
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The synthesis of indole-3-acetyl-1-O-beta-D-glucose from indole-3-acetic acid (IAA) and uridine diphosphoglucose (UDPG) has been shown to be a reversible reaction with the equilibrium away from ester formation and toward formation of IAA. The enzyme occurs primarily in the liquid endosperm of the
Transgenic studies on the involvement of cytokinin and gibberellin in male development.
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Numerous plant hormones interact during plant growth and development. Elucidating the role of these various hormones on particular tissue types or developmental stages has been difficult with exogenous applications or constitutive expression studies. Therefore, we used tissue-specific promoters
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