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13 Ergebnisse
Cell surface carbohydrate of Leishmania mexicana amazonensis: differences between infective and non-infective forms.
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The cell surface carbohydrates of Leishmania mexicana amazonensis (amastigotes and promastigotes, both infective and non-infective forms) were comparatively analyzed by agglutination assay employing 28 highly purified lectins, and by binding assay using 125I-labeled lectins. Among the D-GalNAc
Lectin binding to Renaut bodies.
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Localization of sugar residues of Renaut bodies in human sural nerves was studied using lectin histochemistry. Lens culinaris specific to mannose and Triticum vulgaris specific to glcNAc bound to the capsular and core portions of Renaut bodies strongly. Arachis hypogaea specific to galb(1-3)galNAc
Intracellular lectin-binding sites in symbiont-bearing Crithidia species.
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Crithidia oncopelti, C. deanei, and C. desouzai are flagellates of the Trypanosomatidae family that present bacterium-like endosymbionts in their cytoplasm. Direct and indirect lectin-gold labeling techniques were used at the electron microscopic level in Lowicryl K4M-embedded cells to demonstrate
Histochemistry of glandular metaplasia at the trigone of the urinary bladder in cows.
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The epithelium at the trigone of the urinary bladder showed intestinal metaplasia of a colonic type in three Holstein-Friesian cows affected with chronic polypoid cystitis. Except for Ricinus communis (RCA-I), almost all goblet cells in the whole crypt were positive to periodic acid-Schiff (PAS),
Histochemical study of lectin binding in the human fetal minor salivary glands.
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The emerging synthesis of glycoconjugates containing specific oligosaccharides in developing human fetal labial and lingual salivary glands has been investigated by lectin histochemistry. An avidin-biotin technique was used to study the binding of lectins from Ulex europeus I (UEA-I), Dolichos
Lectin histochemistry of trachea and lung of healthy turkeys (Meleagris gallopavo) and turkeys with pneumonia.
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Thirteen lectins were used to characterize lectin-binding specificity of glycoconjugates on sections of formalin-fixed lung and trachea from seven normal turkeys, two turkeys with acute pneumonia, and two turkeys with chronic pneumonia. Neuraminidase was used to digest sialic acid residues. One
Expression pattern of glycoconjugates in the Bidderian and ovarian follicles of the Brazilian toad Bufo ictericus analyzed by lectin histochemistry.
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The Bidder's organ and ovary of the Brazilian toad Bufo ictericus were studied by light microscopy, using hematoxylin-eosin (HE) and periodic acid Schiff (PAS) staining. The expression and distribution of carbohydrate moieties was analyzed by lectin histochemistry, using 8 lectins with different
Lectin histochemistry of dorsal skin of Wistar-derived hypotrichotic WBN/Ila-Ht rats.
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A lectin histochemical study was carried out on the dorsal skin of Wistar-derived hypotrichotic WBN/Ila-Ht rats (HtRs) and Wistar rats (WRs) at 3, 7 and 24 weeks of age to clarify the lectinhistochemical characteristics of the skin during their development. The lectins examined were Concanavalia
Histochemical study of the epithelia of nasal polyps by biotinylated lectins and neoglycoprotein. A comparison with the normal human respiratory epithelium.
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Formalin-fixed, paraffin-embedded sections from human nasal polyps and the normal respiratory epithelium were glycohistochemically investigated. Three biotinylated lectins were used: peanut (Arachis hypogaea) agglutinin (PNA) which, binds to terminal galactose (beta 1-3) N-acetylgalactosamine
Lectin-dependent attachment of Actinomyces naeslundii to receptors on epithelial cells.
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The adherence of Actinomyces naeslundii WVU45 to monolayer cultures of human epithelial cell lines was mediated by the lactose-sensitive fimbriae (type 2) of strain WVU45. The attachment of Actinomyces viscosus T14V, which has both types 1 and 2 fimbriae, was approximately half that of A.
CBP70, a glycosylated nuclear lectin.
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Some years ago, a lectin designated CBP70 that recognized glucose (Glc) but had a stronger affinity for N-acetylglucosamine (GlcNAc), was first isolated from HL60 cell nuclei. Recently, a cytoplasmic form of this lectin was described, and one 82 kDa nuclear ligand was characterized for the nuclear
Lectin binding affinities of induced pancreatic lesions in the hamster model.
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We examined the binding pattern of nine lectins to N-nitrosobis(2-oxopropyl)amine (BOP)-induced pancreatic lesions in Syrian hamsters. These lectins were Arachis hypogaea (PNA), Dolichos biflorus (DBA), Griffonia simplicifolia I (GS-I), Helix aspersa (HAA), Helix pomatia (HPA), Sophora japonica
Lectin binding and effects in culture on human cancer and non-cancer cell lines: examination of issues of interest in drug design strategies.
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By using a non-cancer and a cancer cell line originally from the same tissue (colon), coupled with testing lectins for cell binding and for their effects on these cell lines in culture, this study describes a simple multi-parameter approach that has revealed some interesting results that could be
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