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Seite 1 von 18 Ergebnisse
Rapid Initiation of Thymidine Incorporation into Deoxyribonucleic Acid in Vegetative Tobacco Stem Segments Treated with Indole-3-acetic Acid.
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The short term effect of 11.4 mum indoleacetic acid on the incorporation of (methyl-(3)H)thymidine into DNA in vegetative tobacco (Nicotiana tabacum cv. Wis. 38) stem segments has been investigated. In segments that are defoliated, inverted, and kept in the dark for 7 hours, indoleacetic acid very
Variable expression of the herpes simplex virus thymidine kinase gene in Nicotiana tabacum affects negative selection.
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The potentials and limitations of negative-selection systems based on the human herpes simplex virus thymidine kinase type-1 (HSVtk) gene, which causes sensitivity to the nucleoside analog ganciclovir, were examined in tobacco as a model system. There were great differences between individual
[ON THE INCORPORATION OF THYMIDINE-H3 IN DNA OF THE CHLOROPLASTS OF NICOTIANA RUSTICA].
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Preferential synthesis of plastid DNA and increased replication of plastids in cultured tobacco cells following medium renewal.
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During the culture of tobacco BY 2 cells derived from Nicotiana tabacum L. cv. Bright Yellow 2, morphological changes of plastid (pt) nucleoids and their replication were examined by fluorescence microscopy after staining with 4'6-diamidino-2-phenylindole. Upon transfer to fresh medium, the
Flower Formation in Excised Tobacco Stem Segments: III. Deoxyribonucleic Acid Content in Stem Tissue of Vegetative and Flowering Tobacco Plants.
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A method has been developed that extracts DNA from stem tissue of flowering tobacco plants, Nicotiana tabacum cv. Wis. 38. The DNA content of stem tissue from a flowering tobacco plant is correlated with its capacity to flower in vitro. Stem segments known to form 100% floral buds contain 10 times
Determination of 5-methylcytosine from plant DNA by high-performance liquid chromatography.
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The relative amounts of the five nucleosides (deoxycytidine, 5-methyldeoxycytidine, deoxyadenosine, deoxyguanosine and thymidine) in the DNA of nine plant species, one plant satellite DNA, and one animal species were determined by high performance liquid chromatography. The method allows the clean
N5-Formyltetrahydrofolate counteracts methotrexate toxicity in tobacco cell culture.
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N5-Formyltetrahydrofolate (leucovorin) counteracted methotrexate toxicity in suspension cultures of Nicotiana tabacum var. Xanthi. Methotrexate at 50 nM inhibited growth of the tobacco cell cultures by 87%. Leucovorin at 200 uM reduced growth inhibition in methotrexate-treated cultures to 37%, but
Transient expression, purification and characterization of bioactive human fibroblast growth factor 8b in tobacco plants.
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cDNA of human fibroblast growth factor 8 isoform b (FGF8b) was cloned for the first time into a plant expression vector with or without endoplasmic reticulum retention signal (KDEL) and was transiently expressed as His tagged fusion protein in Nicotiana tabacum leaves through Agrobacterium mediated
Passage of bacterial DNA into host cells during in vitro transformation of Nicotiana tabacum by Agrobacterium tumefaciens.
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Tumorigenic and non-tumorigenic strains of Agrobacterium tumefaciens were labelled with tritiated thymidine and added separately to suspension cultures of X-D line habituated tobacco cells. The subsequent association of bacteria with plant host cells and the passage of bacteria and bacterial DNA
The G-protein-coupled receptor GCR1 regulates DNA synthesis through activation of phosphatidylinositol-specific phospholipase C.
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Different lines of evidence suggest that specific events during the cell cycle may be mediated by a heterotrimeric G-protein activated by a cognate G-protein coupled receptor. However, coupling between the only known Galpha-subunit of the heterotrimeric G-protein (GPA1) and the only putative
Effect of glyphosate on carrot and tobacco cells.
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The growth of suspension-cultured carrot (Daucus carota L.) and tobacco (Nicotiana tabacum L. cv. Xanthi) cells was inhibited by glyphosate (N-[phosphonomethyl]glycine). This inhibition was reversed by adding combinations of phenylalanine, tyrosine, and tryptophan or casein hydrolysate. Casein
Chloroplast DNA synthesis in light and dark grown cultured Nicotiana tabacum cells as determined by molecular hybridization.
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A simple method using molecular hybridization was devised to quantitatively measure chloroplast DNA synthesis in vivo. Total cellular DNA isolated from Nicotiana tabacum suspension cells, labeled with (3)H-thymidine, was hybridized to nitrocellulose membrane-bound cloned chloroplast DNA (ct DNA)
5-Bromodeoxyuridine: A specific inhibitor of cytokinin-habituation in tobacco cell culture.
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Cytokinin-habituated cells of Nicotiana tabacum L. cv. "Havana 425" are able to grow in culture without added cytokinin. The thymidine analogue, 5-bromodeoxyuridine (BUdR), which selectively inhibits differentiation of animal cells, blocks expression of the cytokinin-habituated phenotype in culture.
EVIDENCE FOR CYTOPLASMIC DNA IN ROOT CELLS OF NICOTIANA.
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Sterile root cultures from Nicotiana tabacum were grown with H(3)-thymidine added to the medium for various intervals. Incorporation of the labeled nucleoside into nuclear DNA occurred in a fraction of the nuclei which increased with time. In addition, the cytoplasm of all cells incorporated enough
Quantitative analysis of the fate of exogenous DNA in Nicotiana protoplasts.
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After a 5-hour incubation of protoplasts of Nicotiana tabacum L. ;Xanthi' with (3)H-DNA (7.26 mug/ml) from N. tabacum L. ;Xanthi nc' 3.5% of the initial radioactivity was found in acid-insoluble substances of the protoplasts. The addition of DEAE-dextran and poly-l-lysine to the incubation medium
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