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tryptophan/sojabohne

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Expression of a feedback insensitive anthranilate synthase gene from tobacco increases free tryptophan in soybean plants.

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Soybean [Glycine max (L.) Merr.] embryogenic cultures were transformed by particle bombardment with the feedback-insensitive tobacco anthranilate synthase (AS) gene ASA2 driven by the CaMV 35S promoter and selected using hph as the selectable marker gene. Only one of eight regenerated lines that set

Plant phenolics affect oxidation of tryptophan.

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The effect of berry phenolics such as anthocyanins, ellagitannins, and proanthocyanidins from raspberry (Rubus idaeus), black currant (Ribes nigrum), and cranberry (Vaccinium oxycoccus) and byproducts of deoiling processes rich in phenolics such as rapeseed (Brassica rapa L.), camelina (Camelina

Characteristics of Modified Leghemoglobins Isolated from Soybean (Glycine max Merr.) Root Nodules.

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Hemoprotein derivatives of an abundant soybean (Glycine max Merr.) root nodule leghemoglobin, Lba, were studied for their modified spectral characteristics and physical properties. Three modified hemoprotein derivatives of Lba (Lbam1, Lbam2, and Lbam3) were purified by preparative isoelectric
BACKGROUND Soybean sprouts (Kongnamool) are one of the most popular and nutritive traditional vegetables in East Asia. Anthracnose caused by Colletotrichum gloeosporioides is one of the most serious diseases of soybean sprouts. In order to obtain basic information for breeding and/or selecting
UV-radiation elicits a suite of developmental (photomorphogenic) and protective responses in plants, but responses early post-germination have received little attention, particularly in intensively bred plants of economic importance. We examined germination, hypocotyl elongation, leaf pubescence and

Effect of chaotropic agents on reversible unfolding of a soybean (Glycine max) seed acid phosphatase.

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In this work we examined the effect of urea and guanidinium chloride on the structural stability of a single isoform of soybean seed acid phosphatase, based on the intensity of tryptophan fluorescence as a function of denaturant concentration. The free energy of unfolding, DeltaGu, was calculated at

Determination of amino acid composition of soybeans (Glycine max) by near-infrared spectroscopy.

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Calibration equations for the estimation of amino acid composition in whole soybeans were developed using partial least squares (PLS), artificial neural networks (ANN), and support vector machines (SVM) regression methods for five models of near-infrared (NIR) spectrometers. The effects of amino

Highly geographical specificity of metabolomic traits among Korean domestic soybeans (Glycine max).

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Classification and characterization of agricultural products at molecular levels are important but often impractical with genotyping, particularly for soybeans that have numerous types of variety and landraces. Alternatively, metabolic signature, a determinant for nutritional value, can be the good

Glyphosate effects on the gene expression of the apical bud in soybean (Glycine max).

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Glyphosate is a broad spectrum, non-selective herbicide which has been widely used for weed control. Much work has focused on elucidating the high accumulation of glyphosate in shoot apical bud (shoot apex). However, to date little is known about the molecular mechanisms of the sensitivity of shoot
Transgenic overexpression of the Arabidopsis gene for jasmonic acid carboxyl methyltransferase (AtJMT) is involved in regulating jasmonate-related plant responses. To examine its role in the compositional profile of soybean (Glycine max), we compared the seeds from field-grown plants that

Indoleacetic Acid synthesis in soybean cotyledon callus tissue.

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Growth of an auxin-requiring soybean cotyledon callus tissue (Glycine max L., Merr. var. Acme) was promoted by tryptophan, tryptamine, indole, indoleacetamide and, to a very slight degree, anthranilic acid. When tryptophan-3-(14)C was supplied in the growth medium, labeled indoleacetic acid (IAA)
The feedback-insensitive anthranilate synthase ( ASA2) cDNA--isolated from a 5-methyltryptophan (5MT)-resistant tobacco cell line--driven by the CaMV 35S promoter or 606 bp of the native ASA2 promoter, was introduced into the forage legume plant Astragalus sinicus or soybean ( Glycine max), using

Circular dichroism studies on the effects of sodium dodecyl sulfate on the conformation of some phytohemagglutinins.

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Conformation and conformational transitions of phytohemagglutinins (lectins) from Arachis hypogaea (peanut), Lens culinaris (lentil), Glycine max (soybean), and Ricinus communis (castor bean) were studied by the circular dichroism probe. The lectins were found to possess a high amount of the pleated

Circular dichroism tests on the effect of alkali on conformation of lectins.

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The structural properties of eight lectins (agglutinins) were studied by the circular dichroism (CD) probe. The lectin from Maclura pomifera (osage orange seed) exhibited in the far-ultraviolet spectral zone a positive CD band centered at 201 nm, whereas the other lectins had positive bands at
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