[Effect of Panax notoginseng saponins on human kidney fibroblast].
Λέξεις-κλειδιά
Αφηρημένη
OBJECTIVE
To study the effect of Panax notoginseng saponins (PNS) on cell proliferation, type I collagen secretion and integrin beta 1 expression in human kidney fibroblast (KFB).
METHODS
KFB were cultured and stimulated by PNS in vitro. The cell proliferation, type I collagen secretion and integrin beta 1 expression in KFB were measured by methyl thiazolyl tetrazolium (MTT), enzyme-linked immunoabsorbent assay (ELISA) and flowcytometry respectively.
RESULTS
Within its optimal concentration range and effective time range, PNS could obviously inhibit the proliferation, type I collagen secretion and integrin beta 1 expression (all P < 0.05) in human KFB.
CONCLUSIONS
PNS would possibly be an effective drug for renal interstitial fibrosis prevention and treatment.