The responses of Arabidopsis thaliana to cadmium exposure explored via metabolite profiling.
Λέξεις-κλειδιά
Αφηρημένη
The metabolic responses of Arabidopsis thaliana to cadmium exposure was characterized in this study. A. thaliana was cultivated in medium contaminated with different cadmium concentrations (0, 5 and 50microM, respectively) for 2weeks. Metabolite analyses were performed using gas chromatography-mass spectrometry. More than 80 metabolites characterized by retention time indices and specific mass fragments were identified. The levels of carbohydrates, organic acids, amino acids, and other stress-responsive metabolites changed under cadmium stress. Treated plants showed increased levels of Ala, beta-ala, Pro, Ser, putrescine, Suc and other metabolites with compatible solute-like properties, notably 4-aminobutyric acid, glycerol, raffinose and trehalose, compared to control (untreated) plants. Studies indicated that concentrations of antioxidants (alfa-tocopherol, campesterol, beta-sitosterol and isoflavone) also increased significantly. These results confirm the important role of antioxidant defences in the mechanisms of plant-resistance to cadmium stress. Our results suggested that metabolic profiling is a powerful tool that can rapidly classify environmentally modified plants and simplify the process of cadmium-stress responses. These data will be helpful for better understanding of mechanisms of plant adaptation to cadmium stress at the metabolite level.