An evaluation of the effects of caffeic acid phenethyl ester and ankaferd blood stopper on secondary wound healing of oral mucosal tissue.

Caffeic acid phenethyl ester (CAPE) and ankaferd blood stopper (ABS) are considered to contribute to wound healing. The purpose of this study was to investigate the effect of ABS and CAPE on secondary wound healing of oral mucosal tissue.In total, 63 male Sprague-Dawley rats were used in this study. The animals were randomly divided into three groups and anesthetized with ketamine (8 mg/100 g, intraperitoneally): Control group, CAPE group, and ABS group. A full-thickness excisional wound was created using a 4 mm punch biopsy tool. Topical ABS and CAPE were then applied in each group for 7, 14, and 21 d (n = 7 in each group). The animals in each group were sacrificed after 7, 14, and 21 d. Palatal specimens were stained with hematoxylin-eosin. Vascular endothelial growth factor (VEGF) and tumor necrosis factor-inducible gene 6 (TSG-6) protein expressions were determined using the western blot method.Inflammation, vessel dilatation, and haemorrhages were significantly lower in the CAPE group as compared with these parameters in the other groups (p < 0.05). Fibrosis was significantly higher in the ABS group as compared with that in the other groups (p < 0.05). VEGF protein levels were elevated in the 21-d CAPE group and 7-d ABS group. The expression of TSG-6 increased in the 7-d CAPE group and 21-d ABS group.Based on our findings. ABS and CAPE had positive effects on the oral wound healing process.