Anti-inflammatory, antioxidant and antiproliferative activities from Trichilia silvatica (C.DC).

The aim of this research was to investigate the anti-inflammatory, antiproliferative and antioxidant activities of the leaves (MEL-TS) and bark (MEB-TS) from T. silvatica. We evaluated the concentrations of phenolic compounds, condensed tannins, flavonoids, flavonols and HPLC/PDA analysis. MEL-TS and MEB-TS were analysed by LC-PDA, and the results revealed the presence of caffeic acid in both extracts. It showed high total phenolics concentrations (270.8 ± 17.10 mg GAE/ g extract and 278.8 ± 25.13 mg GAE/g extract, respectively), and content of flavonoids in MEL-TS (209.30 ± 2.91 mg QE/g extract). In the antioxidant tests, MEL-TS and MEB-TL exhibited moderate scavenging activities with IC50 values ≤ 35.32 μg/mL in lipoperoxidation. Both extracts (30-300 mg/kg) inhibited oedema induced by carrageenan at 2 h and 4 h after carrageenan induction and inhibited leukocyte migration at 6 h post administration. MEL-TS and MEB-TL (100 and 300 mg/kg) did not impact zymosan-induced arthritis in the treated animals. Finally, MEL-TS were particularly effective against VERO and PC-3 cell line resulting in GI50 ≤ 0.22 μg/mL. The results indicated that the leaves and bark from T. silvatica has contributed in part to the traditional use of this genus for the treatment of rheumatism/arthritis.