Anti-inflammatory principles of Suregada multiflora against nitric oxide and prostaglandin E2 releases.
Keywords
Abstract
OBJECTIVE
The stem bark of Suregada multiflora and the isolated compounds were carried out to investigate for anti-inflammatory activity.
METHODS
The stem bark of Suregada multiflora and its isolated compounds were tested for their anti-inflammatory effects against lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostraglandin E(2) (PGE(2)) releases in RAW264.7 cells as well as the anti-inflammatory mechanism on mRNA expression of the active compound (5, helioscopinolide A).
RESULTS
The extract of Suregada multiflora possessed potent NO inhibitory effect with an IC(50) value of 8.6 μg/ml. Among the isolated compounds, helioscopinolide A (5) exhibited the highest activity against NO release with an IC(50) value of 9.1 μM, followed by helioscopinolide C (6) and suremulol D (2) with IC(50) values of 24.5 and 29.3 μM, respectively. The IC(50) value of 5 against PGE(2) production was found to be 46.3 μM. The mechanism in transcriptional level of compound 5 was found to inhibit iNOS and COX-2 mRNA expressions in dose-dependent manners.
CONCLUSIONS
The present study may support the traditional use of Suregada multiflora stem bark for treatment of inflammatory-related diseases.
